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Safety Information

96992

Sigma-Aldrich

Cell Counting Kit - 8

for quantitation of viable cell number in proliferation and cytotoxicity assays

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About This Item

UNSPSC Code:
12352108
NACRES:
NA.32

Quality Level

100

shelf life

limited shelf life, expiry date on the label

shipped in

ambient

storage temp.

2-8°C

Application

Cell Counting Kit-8 (CCK-8) allows convenient assays using WST-8 (2-(2-methoxy-4-nitrophenyl)-3-(4-nitrophenyl)-5-(2,4-disulfophenyl)-2H-tetrazolium, monosodium salt), which produces a water-soluble formazan dye upon bioreduction in the presence of an electron carrier, 1-Methoxy PMS. CCK-8 solution is added directly to the cells, no pre-mixing of components is required. WST-8 is bioreduced by cellular dehydrogenases to an orange formazan product that is soluble in tissue culture medium. The amount of formazan produced is directly proportional to the number of living cells. Since the CCK-8 solution is very stable and it has little cytotoxicity, a longer incubation, such as 24 to 48 hours, is possible.
Cell Counting Kit-8 allows sensitive colorimetric assays for the determination of the number of viable cells in the proliferation and cytotoxicity assays. The detection sensitivity is higher than any other tetrazolium salts such as MTT, XTT or MTS.

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Product No.
Description
Pricing

Pictograms

Health hazardExclamation mark
GHS08,GHS07

Signal Word

Warning

Hazard Statements

H315,H319,H341,H351

Hazard Classifications

Carc. 2 - Eye Irrit. 2 - Muta. 2 - Skin Irrit. 2

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

96992-250TESTS-F:
96992-1KT-BULK-F:
96992-5X600TESTS-F:
96992-100TESTS-BUL:
96992-500TESTS-F:
96992-1EA-BULK-F:
96992-100TESTS-F:
96992-3000TESTS-F:
96992-1KT-F:

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Certificates of Analysis (COA)

Lot/Batch Number
BCCL9232
BCCL2958
BCCL2957
BCCM4061
BCCL2637

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Cell Viability and Proliferation Assays

Cell based assays for cell proliferation (BrdU, MTT, WST1), cell viability and cytotoxicity experiments for applications in cancer, neuroscience and stem cell research.

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