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A2580

Sigma-Aldrich

Angiotensin Converting Enzyme from porcine kidney

lyophilized powder, ≥10 units/mg protein (Bradford)

Synonym(s):

ACE, Peptidyl-dipeptidase A

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About This Item

CAS Number:
9015-82-1
Enzyme Commission number:
3.4.15.1 (BRENDA, IUBMB)
MDL number:
MFCD00130325
UNSPSC Code:
12352200
NACRES:
NA.32

form

lyophilized powder

Quality Level

200

specific activity

≥10 units/mg protein (Bradford)

UniProt accession no.

A0A480IQM9

shipped in

dry ice

storage temp.

−20°C

Gene Information

pig ... ACE(613133)

General description

ACE is a monomer with molecular weight of ~170 kDa
pH range for activity: 7-8.5
Temperature optimum: 37 °C
Zinc is required for activity
Inhibitors: captopril, enalaprilat, lisinopril etc. (1-10 μM)
Angiotensin converting enzyme (ACE) is encoded by the gene, mRNA-decapping enzyme subunit 1 (DCP1). It is a zinc metalloprotease, which belongs to the M2 family.

Application

Angiotensin converting enzyme from porcine kidney has been used in in vitro angiotensin-converting enzyme (ACE) inhibition assay.

Biochem/physiol Actions

Angiotensin converting enzyme (ACE) catalyzes the conversion of angiotensin I to angiotensin II, which regulates the fluid-electrolyte balance and systemic blood pressure. ACE inhibits the vasodilator, bradykinin. ACE inhibitors are used to treat high blood pressure.
Removes C-terminal dipeptides from susceptible substrates, e.g., angiotensin I and bradykinin.

Unit Definition

One unit will produce 1.0 ·μmole of hippuric acid from Hippuryl-His-Leu per min in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 °C.

Physical form

Lyophilized powder containing Tris buffer salts.

inhibitor

Product No.
Description
Pricing

substrate

Product No.
Description
Pricing

Pictograms

Health hazard
GHS08

Signal Word

Danger

Hazard Statements

H334

Precautionary Statements

P261 - P284 - P501

Hazard Classifications

Resp. Sens. 1

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

A2580-.5UN:
A2580-.1UN:
A2580-.5UN-PW:
A2580-1UN-PW:
A2580-BULK:
A2580-.1UN-PW:
A2580-PH:
A2580-1UN:
A2580-VAR:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCQ6821
SLCP1569
SLBQ3761V
SLBD9803V
050M3779

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Sequence variation in the human angiotensin converting enzyme
Rieder MJ, et al.
Nature Genetics, 22(1), 59-59 (1999)
Effect of angiotensin-converting-enzyme inhibition compared with conventional therapy on cardiovascular morbidity and mortality in hypertension: the Captopril Prevention Project (CAPPP) randomised trial
Hansson L, et al.
Lancet, 353(9153), 611-616 (1999)
Evaluation of mechanism for antihypertensive action of Clerodendrum colebrookianum Walp., used by folklore healers in north-east India
Lokesh D and Amitsankar D
Journal of Ethnopharmacology, 143(1), 207-212 (2012)
N M Hooper et al.
The Biochemical journal, 241(3), 625-633 (1987-02-01)
Peptidyl-dipeptidase A (angiotensin converting enzyme; ACE, EC 3.4.15.1), has been purified from pig kidney and striatum by affinity chromatography employing the selective inhibitor lisinopril as ligand. The inclusion of a 2.8 nm spacer arm improved the yield of the enzyme
Prolyl carboxypeptidase: a forgotten kidney angiotensinase. Focus on "Identification of prolyl carboxypeptidase as an alternative enzyme for processing of renal angiotensin II using mass spectrometry".
Juan Carlos Q Velez
American journal of physiology. Cell physiology, 304(10), C939-C940 (2013-04-05)
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