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C8554

Sigma-Aldrich

Chorionic gonadotropin human

lyophilized powder, suitable for mouse embryo cell culture

Synonym(s):

Choriogonin, HCG

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About This Item

CAS Number:
9002-61-3
EC Number:
232-660-2
MDL number:
MFCD00130797
UNSPSC Code:
12352202
NACRES:
NA.32
biological source:
human urine
form:
lyophilized powder

biological source

human urine

Quality Level

200

sterility

aseptically processed

form

lyophilized powder

potency

≥5,000 I.U. per mg

mol wt

37.9 kDa
apparent mol wt 37.9 kDa by SDS-PAGE

drug control

Home Office Schedule 4.2

technique(s)

cell culture | embryo: suitable

impurities

HBV,HCV and HIV, tested negative

solubility

H2O: 1 mL/vial
saline: 1 mL/vial

UniProt accession no.

P01215

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... CGA(1081)

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General description

Glycoprotein hormone consisting of a 92-amino acid α-chain which is identical to that of LH, FSH, and TSH; and a distinct 149-amino acid β-chain. Produced by chorionic tissue and responsible for maintaining the corpus luteum during early pregnancy. Also produced by trophoblast cell neoplasms, which are marked by elevated free hCG β-chains and hyperglycosylated hCG.
Note: Actual molecular weight will vary from the theoretical depending upon the degree of glycosylation. Theoretical MW is 37.9 kDa based on native form which contains 2 subunits: α = 14.9 kDa (~10.2 kDa for the polypeptide and ~4.7 kDa for the carbohydrate), and β = 23.0 kDa (~16.0 kDa for the polypeptide and ~7.0 kDa for the carbohydrate). See J. Biol. Chem. 248, 6796-6827 (1973).

Application

Recommended for superovulation induction in mice.

Biochem/physiol Actions

Human chorionic gondadotropin (hCG) may be used as an alternative to PMSG to induce ovulation in laboratory animals. When hCG was injected into laboratory animals via intravenous (i.v.), intraperitoneal (i.p.), or subcutaneous (s.c.) methodstet, the effective dose values for 50% of the animals (ED50) per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.2 (7.7) I.U.; i.p. 0.3 (11.5) I.U.; s.c. 0.7 (26.9) I.U.
Syrian hamsters: i.v. 1.0 (9.5) I.U.; i.p. 1.8 (17.1) I.U.; s.c. 2.6 (24.8) I.U.
Rats: i.v. 1.3 (4.6) I.U.; i.p. 3.5 (12.3) I.U.; s.c.7.5 (26.3) I.U.

Using PMSG, the ED50 values per animal and per body weight (kg) in parenthesis were as follows:
Mice: i.v. 0.8 (30.8) I.U.; i.p. 2.0 (76.9) I.U.; s.c. 2.8 (107.7) I.U
Syrian hamsters: i.v., 3.6 (34.3) I.U.; i. p., 8.0 (76.2) I.U.; s.c., 13.2 (125.7) I.U.
Rats: i.v., 6.0 (76.8) I.U.; i p., 20.8 (73.0) I.U.; s.c., 76.8 (269.5) I.U.

Pictograms

Health hazard
GHS08

Signal Word

Warning

Hazard Statements

H361

Precautionary Statements

P281

Hazard Classifications

Repr. 2

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

C8554-50IU-PW:
C8554-VAR:
C8554-50IU:
C8554-BULK:
C8554-PH:

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Certificates of Analysis (COA)

Lot/Batch Number
SLBS2471V
SLBD8153
109K8708
097K8629
124K8925

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