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H5505

Sigma-Aldrich

Histone from calf thymus

Type III-S

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About This Item

CAS Number:
9064-47-5
Beilstein:
1895465
MDL number:
MFCD00131329
UNSPSC Code:
41106305
NACRES:
NA.51

biological source

bovine (calf) thymus

Quality Level

200

type

Type III-S

form

powder

concentration

≥60% protein (biuret)

technique(s)

activity assay: suitable

solubility

H2O: soluble 10 mg/mL, clear to hazy, colorless to light yellow

storage temp.

2-8°C

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Application

Histone is suitable for use:
  • as a positive control in the in vitro kinase assay to examine the ability of Cdc2-cyclin B1 to phosphorylate GST-CD-4 protein
  • in Cdk5 activity assay
  • in histone H1 kinase assay
  • as a Poly(ADP-ribose) (PAR) acceptor in the assessment of in vitro PAR polymerase activity
  • as an acceptor of 32P from [γ-32P]ATP in order to measure protein kinase activity of lysate from small and large luteal cells

Biochem/physiol Actions

Histones are a group of proteins characterized by high levels of lysine and arginine that form reversible complexes with DNA. Based on the fractions, the molar mass of histones varies between 11-21kDa. Five different fractions have been isolated and characterized. They include H1 (lysine rich), H2a and H2b that are slightly lysine rich, H3 and H4 that are arginine rich. H1 acts as a link between "beads" (nu bodies) on the chromatin chain.

Features and Benefits

Do not confuse Sigma "Type" designations with the "Fraction" designations used by Luck and co-workers.

Other Notes

Characterized as mainly subgroup f1 by SDS-PAGE

Preparation Note

Lysine-rich fraction as isolated and described by de Nooij, E. and Westenbrink, H.G., Biochim. Biophys. Acta, 62, 608 (1962).

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

H5505-VAR:
H5505-100MG:
H5505-250MG:
H5505-BULK:
H5505-25MG:

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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