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Safety Information

SRP0217

Sigma-Aldrich

SHP-2 Active human

recombinant, expressed in E. coli, ≥90% (SDS-PAGE)

Synonym(s):

Noonan syndrome 1, PTP-1D, PTP2C, PTPN11, SHP2

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About This Item

UNSPSC Code:
12352200
NACRES:
NA.32

biological source

human

recombinant

expressed in E. coli

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

59.5 kDa

packaging

pkg of 20 μg

storage condition

avoid repeated freeze/thaw cycles

concentration

0.73 mg/mL

NCBI accession no.

NM_002834

UniProt accession no.

Q06124

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... PTPN11(5781)

General description

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11), also known as SHP-2, is a scaffolding protein. It possesses two SH2 domains at the amino terminal, a protein tyrosine phosphatase domain and carboxy-terminal region. The gene encoding SHP-2 is localized on human chromosome 12q24.13.

Application

Useful for the study of enzyme kinetics, regulation, to dephosphorylate target substrates and for screening inhibitors.
Human active SHP-2 has been used to study the effects of α-lipoic acid and dihydrolipoic acid on the activity of SHP-2 enzyme.

Biochem/physiol Actions

Protein tyrosine phosphatase, non-receptor type 11 (PTPN11) or SHP-2 is involved in signal transduction pathways downstream of growth receptors. It acts as a proto-oncogene. SHP-2 leads to sustained RAS activation by dephosphorylating the RAS-GTPase-activating protein binding site on Grb2-associated binder 1 (Gab1). This protein also nucleates multiple signaling proteins and thus, acts as an adaptor protein. Mutations in the SHP-2 gene have been associated with Noonan syndrome.

Unit Definition

One unit will hydrolyze 1 nmol p-nitrophenyl phosphate per minute at pH 7.4 and 30°C.

Physical form

Formulated in 25 mM Tris-HCl, pH 8.0, 75 mM NaCl, 0.05% Tween-20, 50% glycerol, 2 mM EDTA, 1 mM DTT, and 10 mM glutathione.

Preparation Note

Thaw on ice. Upon first thaw, briefly spin tube containing enzyme to recover full content of the tube. Aliquot enzyme into single use aliquots. Store remaining undiluted enzyme in aliquots at -70°C. Note: Enzyme is very sensitive to freeze/thaw cycles.

Other Notes

Amino acids : 224-529

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

SRP0217-20UG:
SRP0217-50UG:

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Certificates of Analysis (COA)

Lot/Batch Number
3002230807
30022090608
3002290608

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Hotspots in PTPN11 Gene Among Indian Children With Noonan Syndrome.
Narayanan DL
Indian Pediatrics, 54(8), 638-643 (2017)
Lipoic Acid Decreases the Viability of Breast Cancer Cells and Activity of PTP1B and SHP2
Anticancer Research, 37(6) (2017)
Shp2 Plays a Critical Role in IL-6-Induced EMT in Breast Cancer Cells.
Sun X
International Journal of Molecular Sciences, 18(2) (2017)
Genomic Duplication of PTPN11 is an Uncommon Cause of Noonan Syndrome
John M
American Journal of Medical Genetics. Part A (2009)
Naphak Modhiran et al.
Science translational medicine, 7(304), 304ra142-304ra142 (2015-09-12)
Complications arising from dengue virus infection include potentially fatal vascular leak, and severe disease has been linked with excessive immune cell activation. An understanding of the triggers of this activation is critical for the development of appropriately targeted disease control
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