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Safety Information

U9257

Sigma-Aldrich

UbcH9 Conjugating Enzyme human

≥95% (SDS-PAGE), recombinant, expressed in E. coli, buffered aqueous glycerol solution

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About This Item

MDL number:
MFCD03458912
UNSPSC Code:
12352202
NACRES:
NA.56

recombinant

expressed in E. coli

Quality Level

200

Assay

≥95% (SDS-PAGE)

form

buffered aqueous glycerol solution

UniProt accession no.

P63279
Q969T4

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... UBE2E3(10477) , UBE2I(7329)

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General description

Manufactured for Sigma by Boston Biochem., Inc.

Biochem/physiol Actions

UbcH9 mediates the conjugation of SUMO-1, SUMO-2, and SUMO-3 to a diverse variety of proteins without the help of an E3 ligase. This E2 conjugating enzyme interacts directly with protein substrates that are modified by SUMOylation, and may help in substrate recognition. UbcH9 is specific for the conjugation of proteins modified by SUMOylation.

Physical form

Solution in 50 mM HEPES, pH 7.5, 100 mM NaCl, 10 % glycerol, 1mM TCEP.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Regulatory Listings

Regulatory Listings are mainly provided for chemical products. Only limited information can be provided here for non-chemical products. No entry means none of the components are listed. It is the user’s obligation to ensure the safe and legal use of the product.

JAN Code

U9257-BULK:
U9257-VAR:
U9257-100UG-PW:
U9257-100UG:

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Certificates of Analysis (COA)

Lot/Batch Number
SLCC2265
SLCD0217
SLBK8303V
078K0555
028K0572

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J M Desterro et al.
FEBS letters, 417(3), 297-300 (1997-12-31)
Ubiquitin conjugating enzymes participate in the thioester cascade that leads to protein ubiquitination. Although Ubc9 is homologous to E2 ubiquitin conjugating enzymes we have shown that it is unable to form a thioester with ubiquitin, but can form a thioester

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