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  • Production Optimization, Structural Analysis, and Prebiotic- and Anti-Inflammatory Effects of Gluco-Oligosaccharides Produced by Leuconostoc lactis SBC001.

Production Optimization, Structural Analysis, and Prebiotic- and Anti-Inflammatory Effects of Gluco-Oligosaccharides Produced by Leuconostoc lactis SBC001.

Microorganisms (2021-01-23)
Minhui Kim, Jae-Kweon Jang, Young-Seo Park
要旨

Leuconostoc lactis SBC001, isolated from chive, produces glucansucrase and synthesizes oligosaccharides through its enzymatic activity. This study was conducted to optimize oligosaccharide production using response surface methodology, analyze the structure of purified oligosaccharides, and investigate the prebiotic effect on 24 bacterial and yeast strains and the anti-inflammatory activity using RAW 264.7 macrophage cells. The optimal conditions for oligosaccharide production were a culture temperature of 30 °C and sucrose and maltose concentrations of 9.6% and 7.4%, respectively. Based on 1H-NMR spectroscopic study, the oligosaccharides were identified as gluco-oligosaccharides that consisted of 23.63% α-1,4 glycosidic linkages and 76.37% α-1,6 glycosidic linkages with an average molecular weight of 1137 Da. The oligosaccharides promoted the growth of bacterial and yeast strains, including Lactobacillus plantarum, L. paracasei, L. johnsonii, Leuconostoc mesenteroides, L. rhamnosus, and Saccharomyces cerevisiae. When lipopolysaccharide-stimulated RAW 264.7 cells were treated with the oligosaccharides, the production of nitric oxide was decreased; the expression of inducible nitric oxide synthase, tumor necrosis factor-α, interleukin (IL)-1β, IL-6, and IL-10 was suppressed; and the nuclear factor-kappa B signaling pathway was inhibited. In conclusion, the gluco-oligosaccharides obtained from Leu. lactis SBC001 exhibited a prebiotic effect on six bacterial and yeast strains and anti-inflammatory activity in RAW 264.7 macrophage cells.

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製品内容

Sigma-Aldrich
α-アミラーゼ from Aspergillus oryzae, ≥150 units/mg protein (biuret)
Sigma-Aldrich
アミログルコシダーゼ from Aspergillus niger, lyophilized powder, 30-60 units/mg protein (biuret), ≤0.02% glucose
Sigma-Aldrich
β-グルコシダーゼ from almonds, lyophilized powder, ≥2 units/mg solid
Sigma-Aldrich
フルクトオリゴ糖 from chicory, ≥90% (as FOS and inulin)
Sigma-Aldrich
α-グルコシダーゼ Bacillus stearothermophilus由来, lyophilized powder, ≥50 units/mg protein
Sigma-Aldrich
β-(1→3)-D-グルカナーゼ from Helix pomatia, ≥0.2 U/mg
Sigma-Aldrich
(Tyr[SO3H]27)コレシストキニンフラグメント 26-33 アミド, ≥97% (HPLC), powder
Sigma-Aldrich
リポポリサッカリド 大腸菌K-235由来, purified by gel-filtration chromatography