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Key Documents

L2018

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli K-235

purified by gel-filtration chromatography

Synonym(s):

LPS

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About This Item

EC Number:
297-473-0
MDL number:
MFCD00164401
UNSPSC Code:
12352201
NACRES:
NA.25

biological source

Escherichia coli (K-235)

Quality Level

200

form

lyophilized powder

purified by

gel-filtration chromatography

impurities

<1% Protein

color

white to yellow cast

solubility

water: 4.90-5.10 mg/mL, cloudy to hazy, colorless to faintly yellow

shipped in

ambient

storage temp.

2-8°C

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General description

This product is extracted from E. coli serotype K235 and purified by gel filtration. The source strain is ATCC 13027.

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The product is soluble in water (5 mg/ml) or cell culture medium (1 mg/ml) yielding a hazy, faint yellow solution. A more concentrated, though still hazy, solution (20 mg/ml) has been achieved in aqueous saline after vortexing and warming to 70-80 oC. Lipopolysaccharides are molecules that form micelles in every solvent. Hazy solutions are observed in water and phosphate buffered saline. Organic solvents do not give clearer solutions. Methanol yields a turbid suspension with floaters, while water yields a homogeneously hazy solution.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

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pictograms

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hcodes

H300

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

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Peipei Zhang et al.
Science advances, 7(43), eabi9471-eabi9471 (2021-10-23)
[Figure: see text].
Waleed Al Abdulmonem et al.
Journal of reproductive immunology, 141, 103152-103152 (2020-06-11)
During first trimester of human pregnancy, the maternal system develops immunity against infection and to provide protection of allogeneic foetus from abortion. This study was undertaken to determine the role of trophoblast specific CD74 isoforms in first trimester trophoblast derived
Jonathan J Hansen et al.
Inflammatory bowel diseases, 15(6), 890-899 (2009-01-13)
While others have described gene expression patterns in humans with inflammatory bowel diseases and animals with chemically induced colitis, a genome-wide comparison of gene expression in genetically susceptible animals that develop spontaneous colitis has not been reported. We used microarray
Tao Ni et al.
Science advances, 6(5), eaax8286-eaax8286 (2020-02-18)
Perforin-2 (MPEG1) is thought to enable the killing of invading microbes engulfed by macrophages and other phagocytes, forming pores in their membranes. Loss of perforin-2 renders individual phagocytes and whole organisms significantly more susceptible to bacterial pathogens. Here, we reveal
Arjen van den Berg et al.
Respiratory research, 6, 111-111 (2005-09-28)
The adenoviral protein E1A has been proposed to play a role in the pathophysiology of COPD, in particular by increasing IL-8 gene transcription of lung epithelial cells in response to cigarette smoke-constituents such as LPS. As IL-8 production is also
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Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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