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L6529

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

γ-irradiated, BioXtra, suitable for cell culture

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Synonym(s):
LPS
EC Number:
MDL number:

biological source

Escherichia coli (O55:B5)

Quality Level

sterility

γ-irradiated

product line

BioXtra

form

lyophilized powder

purified by

gel-filtration chromatography

technique(s)

cell culture | mammalian: suitable

impurities

<3% Protein (Lowry)

solubility

H2O: 5 mg/mL, faintly hazy to hazy

storage temp.

2-8°C

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1 of 4

This Item
L4005F8666L4391
vibrant-m

L6529

Lipopolysaccharides from Escherichia coli O55:B5

Premium Grade
vibrant-m

L4391

Lipopolysaccharides from Escherichia coli O111:B4

Premium Grade
biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O55:B5)

biological source

Escherichia coli (O111:B4)

solubility

H2O: 5 mg/mL, faintly hazy to hazy

solubility

water: soluble

solubility

H2O: 4.90-5.10 mg/mL, faintly hazy to hazy, pale yellow to yellow

solubility

H2O: 5 mg/mL, slightly hazy

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Quality Level

300

Quality Level

200

Quality Level

200

Quality Level

300

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

General description

Lipopolysaccharides (LPS) are complex glycolipids that contain several components. Enterobacteriaceae, such as E. coli, have highly conserved lipid A linked to the antigenic O-polysaccharide. Hydrophobic lipid A forms the outer most component that plays a role in the biological properties attributed to endotoxic LPS. A short core oligosaccharide (core OS) is found to be attached to lipid A. Enterobacteria contain an antigenic, repeat-structure O-polysaccharide (O-PS) attached to the distal core OS to form smooth LPS. O-PS in E. coli contains ∼170 distinct antigens that interact with the host immune system. Based on the core OS structures in E. coli, they have been designated K-12 and R1 to R4.

application

LPS (lipopolysaccharide) from Escherichia coli 055:B5 has been used:
  • to stimulate human PBMC to secrete cytokine.
  • to stimulate wild type embryonic fibroblasts and mutants for the induction of phosphorylation of p56
  • in the measurement of tumor necrosis factor-α levels and nuclear factor-κB p65 activities after stimulation
  • in the stimulation of hamster lymphocytes in vitro
  • to induce activated dendritic morphology in murine macrophage cell line in a study
Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

LPS (lipopolysaccharide) is a major constituent of the outer membrane of most gram negative bacteria. It is a highly immunogenic antigen with the ability to enhance immune responses to soluble antigens. The LPS confers antigenic and endotoxic characters to the bacteria. They are also important in resistance to infection by phages.

Reconstitution

Lipopolysaccharides are supplied as lyophilized, γ-irradiated powders. To reconstitute, add 1 ml sterile balanced salt solution or tissue culture medium to the vial (1 mg) and gently swirl until the powder dissolves. Reconstituted product may be further diluted to desired working concentrations using sterile balanced salt solution or tissue culture medium.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

pictograms

Skull and crossbones

signalword

Danger

hcodes

Hazard Classifications

Acute Tox. 2 Oral

Storage Class

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Cheng Yang Ng et al.
Scientific reports, 7(1), 13683-13683 (2017-10-24)
Cytosolic phospholipase A
PURIFICATION AND CHARACTERIZATION OF THE LIPID A COMPONENT OF THE LIPOPOLYSACCHARIDES FROM ESCHERICHIA COLI.
A J BURTON et al.
Biochemistry, 3, 411-418 (1964-03-01)
Christopher D Hammerbeck et al.
Journal of virology, 85(19), 9929-9944 (2011-07-22)
Andes virus (ANDV) is associated with a lethal vascular leak syndrome in humans termed hantavirus pulmonary syndrome (HPS). In hamsters, ANDV causes a respiratory distress syndrome closely resembling human HPS. The mechanism for the massive vascular leakage associated with HPS
Natalie M Otto et al.
Critical care (London, England), 12(4), R107-R107 (2008-08-20)
Hyperglycemia is associated with negative outcomes in various settings of critical illness; infectious complications, especially, seem to be increased. On the other hand, intensive insulin therapy (IIT) has been shown to improve outcome in clinical trials. Whether normoglycemia itself or
K Amor et al.
Infection and immunity, 68(3), 1116-1124 (2000-02-26)
In the lipopolysaccharides of Escherichia coli there are five distinct core oligosaccharide (core OS) structures, designated K-12 and R1 to R4. The objective of this work was to determine the prevalences of these core OS types within the species. Unique

Articles

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

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