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L5418

Sigma-Aldrich

Lipopolysaccharides from Escherichia coli O55:B5

Ready Made solution, 1 mg/mL

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Synonym(s):
LPS
MDL number:

biological source

Escherichia coli (O55:B5)

Quality Level

form

aqueous solution

concentration

1 mg/mL

color

white to faint yellow

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

shipped in

wet ice

storage temp.

2-8°C

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This Item
L4524L6529L4005
vibrant-m

L6529

Lipopolysaccharides from Escherichia coli O55:B5

Premium Grade
Quality Level

200

Quality Level

200

Quality Level

300

Quality Level

200

solubility

water: 4.90-5.10 mg/mL, faintly hazy to hazy, colorless to faintly yellow

solubility

water: soluble

solubility

H2O: 5 mg/mL, faintly hazy to hazy

solubility

water: soluble

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

form

aqueous solution

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

color

white to faint yellow

color

white to yellow cast

color

-

color

white to yellow

General description

This product is a 0.2 um filtered aqueous solution containing 1 mg/mL of lipopolysaccharides (LPS) from E. coli serotype O55:B5. The source strain is CDC 1644-70. This LPS serotype has been used in studies of septic shock and to induce myocarditis.

Application

Lipopolysaccharides (LPSs) are characteristic components of the cell wall of Gram-negative bacteria. LPS and its lipid A moiety stimulate cells of the innate immune system by the Toll-like receptor 4 (TLR4), a member of the Toll-like receptor protein family, which recognizes common pathogen-associated molecular-patterns (PAMPs).

Biochem/physiol Actions

Lipopolysaccharides (LPS) are localized in the outer layer of the membrane and are, in noncapsulated strains, exposed on the cell surface. They contribute to the integrity of the outer membrane, and protect the cell against the action of bile salts and lipophilic antibiotics.

Preparation Note

The LPS used to prepare the solution have been phenol-extracted and chromatographically purified by gel filtration. Lipopolysaccharides prepared by phenol extraction contain up to 60% RNA and less than 1% protein. Subsequent purification by gel filtration chromatography removes much of protein present in the phenol-extracted LPS, but leaves a product that still contains 10-20% nucleic acids. Further purification using ion exchange chromatography yields an LPS product which contains <1% protein and <1% RNA.

Other Notes

To gain a comprehensive understanding of our extensive range of Lipopolysaccharides for your research, we encourage you to visit our Carbohydrates Category page.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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Articles

Explore the structure, function, and diverse applications of Lipopolysaccharides. Discover their role in bacteria, serological specificity, and research potential.

Related Content

Lipopolysaccharide (LPS) is the major component of the outer membrane of Gram-negative bacteria. Lipopolysaccharide is localized in the outer layer of the membrane and is, in noncapsulated strains, exposed on the cell surface.

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