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A1420

Sigma-Aldrich

Ames′ Medium

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

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200 μL
$620.00

$620.00

Estimated to ship onMay 12, 2025

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200 μL
$620.00

About This Item

UNSPSC Code:
41161501
NACRES:
NA.75

$620.00

Estimated to ship onMay 12, 2025

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

components

glucose: 1.081 g/L (Dextro)
NaHCO3: no
L-glutamine: 0.073 g/L

shipped in

ambient

storage temp.

2-8°C

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Monoclonal Anti-Rabbit Immunoglobulins–Biotin antibody produced in mouse

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

species reactivity

rabbit

biological source

mouse

biological source

mouse

biological source

goat

biological source

mouse

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

biotin conjugate

technique(s)

immunocytochemistry: suitable, western blot: 1:10,000-1:20,000 using 3T3 cell extracts using Anti-Actin (20-33), Catalog Number A5060, as the primary antibody, indirect ELISA: suitable, western blot: 1:10,000-1:20,000 using Hela cell extracts using Anti-Actin, N-terminal, Catalog Number A2103, as the primary antibody.

technique(s)

direct ELISA: 1:40,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot: 1:160,000 using total cell extract of HeLa cells (5-10 μg/well)

technique(s)

direct ELISA: 1:30,000 using using 5 μg/ml of rabbit IgG for the coating and OPD substrate, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:200, western blot (chemiluminescent): 1:80,000-1:160,000 using detecting β-actin in total cell extract of HeLa cells (5-10 μg/mL)

technique(s)

direct ELISA: 1:60,000, immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:1,500, western blot: 1:200,000-1:400,000 using using an assay detecting actin in total cell extract of HeLa cells (5-10 μg per well)

isotype

IgG1

isotype

IgG1

isotype

-

isotype

IgG1

General description

Ames′ Medium has been formulated to support retinal tissue in relatively short-term culture. It is suitable for maintaining central nervous system tissue in vitro.

Application

Ames′ Medium has been used to maintain rat/mice/macaque monkey eye for the isolation of retina.[1][2][3]

Quantity

Formulated to contain 8.8 grams of powder per liter of medium.

Reconstitution

Supplement with 1.9 g/L sodium bicarbonate.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

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    Certificates of Analysis (COA)

    Lot/Batch Number
    0000416084
    0000416084
    0000415646
    0000415646
    0000350103

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    Frans Vinberg et al.
    Vision research, 101, 108-117 (2014-06-25)
    The Function of the retina and effects of drugs on it can be assessed by recording transretinal voltage across isolated retina that is perfused with physiological medium. However, building ex vivo ERG apparatus requires substantial amount of time, resources and
    Qiuju Jiang et al.
    IEEE transactions on neural systems and rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society, 26(5), 969-976 (2018-05-13)
    Significant progress has been made recently in treating neurological blindness using implantable visual prostheses. However, implantable medical devices are highly invasive and subject to many safety, efficacy, and cost issues. The discovery that ultrasound (US) may be useful as a
    Antoine Chaffiol et al.
    Molecular therapy : the journal of the American Society of Gene Therapy, 25(11), 2546-2560 (2017-08-16)
    The majority of inherited retinal degenerations converge on the phenotype of photoreceptor cell death. Second- and third-order neurons are spared in these diseases, making it possible to restore retinal light responses using optogenetics. Viral expression of channelrhodopsin in the third-order
    Michael B Manookin et al.
    The Journal of neuroscience : the official journal of the Society for Neuroscience, 28(16), 4136-4150 (2008-04-18)
    Cone signals divide into parallel ON and OFF bipolar cell pathways, which respond to objects brighter or darker than the background and release glutamate onto the corresponding type of ganglion cell. It is assumed that ganglion cell excitatory responses are
    Sabrina Asteriti et al.
    Visual neuroscience, 34, E006-E006 (2017-10-03)
    Rod-cone gap junctions mediate the so-called "secondary rod pathway", one of three routes that convey rod photoreceptor signals across the retina. Connexin 36 (Cx36) is expressed at these gap junctions, but an unidentified connexin protein also seems to be expressed.
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