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W4125

Sigma-Aldrich

Williams′ Medium E

With L-glutamine, without sodium bicarbonate, powder, suitable for cell culture

Synonym(s):

Medium E, Williams E Medium, Williams’ E medium

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About This Item

UNSPSC Code:
12352207
NACRES:
NA.75

form

powder

technique(s)

cell culture | mammalian: suitable

components

sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L
glucose: 2.0 g/L
phenol red: 0.0107 g/L
NaHCO3: no

shipped in

ambient

storage temp.

2-8°C

General description

Williams′ E medium is a rich medium developed by Williams′ and Gunn. It is recommended for supporting long-term adult liver epithelial cell culture.

Application

Williams′ E medium has been used in culturing mice/rat primary hepatocytes.

Quantity

Formulated to contain 10.8 grams of powder per liter of medium.

Reconstitution

Supplement with 2.2 g/L sodium bicarbonate.

also commonly purchased with this product

Product No.
Description
Pricing

supplement

Product No.
Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

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M Herranz-Itúrbide et al.
Redox biology, 40, 101841-101841 (2021-01-26)
Liver is a unique organ in displaying a reparative and regenerative response after acute/chronic damage or partial hepatectomy, when all the cell types must proliferate to re-establish the liver mass. The NADPH oxidase NOX4 mediates Transforming Growth Factor-beta (TGF-β) actions
Etienne Guillocheau et al.
The Journal of nutritional biochemistry, 63, 19-26 (2018-10-14)
Trans-palmitoleic acid (trans-C16:1 n-7 or trans-Δ9-C16:1, TPA) is believed to improve several metabolic parameters according to epidemiological data. TPA may mainly come from direct intakes: however, data are inconsistent due to its very low amount in foods. Instead, TPA might
D M Toivola et al.
Journal of cell science, 110 ( Pt 1), 23-33 (1997-01-01)
The importance of protein phosphatases in the maintenance of cytoskeletal structure is supported by the serious liver injury caused by microcystin-LR, a hepatotoxic inhibitor of type-1 and type-2A serine/threonine protein phosphatases. We used the microcystin-LR-induced cell injury as a model
Yingjiang Zhou et al.
The Journal of biological chemistry, 284(17), 11152-11159 (2009-03-05)
Major urinary protein (MUP) 1 is a lipocalin family member abundantly secreted into the circulation by the liver. MUP1 binds to lipophilic pheromones and is excreted in urine. Urinary MUP1/pheromone complexes mediate chemical communication in rodents. However, it is unclear
Christina P Keravnou et al.
Ultrasound in medicine & biology, 42(11), 2676-2686 (2016-08-25)
Localized drug delivery and uptake can benefit from the combined action of ultrasound and microbubbles at a specific site. Some of the possible mechanisms suggested are vessel poration and/or cell poration, but the exact acoustic parameters that trigger those phenomena
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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