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L4386

Sigma-Aldrich

L-15 Medium (Leibovitz)

With ʟ-glutamine and sodium pyruvate, powder, suitable for cell culture

Synonym(s):

Leibovitz L15

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About This Item

MDL number:
MFCD00217482
UNSPSC Code:
12352207
NACRES:
NA.75

product name

L-15 Medium (Leibovitz), With L-glutamine, powder, suitable for cell culture

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: 0.55 g/L
phenol red: 0.011 g/L
L-glutamine: 0.3 g/L

shipped in

ambient

storage temp.

2-8°C

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General description

L-15 Medium (Leibovitz) is formulated for use in carbon dioxide (CO2) free systems requiring sodium bicarbonate supplements. It supports established cell lines, such as human larynx epidermoid cells (HEp-2) and Rhesus monkey kidney epithelial cells (LLC-MK2), as well as primary explants of embryonic and adult human tissue. L-15 medium can also be used for culturing viruses.

Application

L-15 Medium (Leibovitz) has been used:
  • to study the toxicity of silver nanoparticles (AgNPs) with primary cultures of Eisenia fetida coelomocytes
  • to culture crab hepatopancreas and ovary tissues
  • as a component of the dissociation medium for infusion of the pancreas of mice

Quantity

Formulated to contain 13.8 grams of powder per liter of medium.

pictograms

Corrosion
GHS05

signalword

Danger

hcodes

H315,H318

Hazard Classifications

Eye Dam. 1 - Skin Irrit. 2

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

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Y Qian et al.
Insect biochemistry and molecular biology, 27(5), 387-395 (1997-05-01)
Prostaglandins of the 2-series (e.g. PGE2) are typically synthesized from arachidonic acid (AA) after AA is released from cellular phospholipids after activation of an intracellular phospholipase A2 (PLA2). Treatment of isolated salivary glands with PLA2 inhibitor oleyloxyethyl phosphorylcholine (OPC) or
Steven J Conrad et al.
Avian diseases, 64(3), 243-246 (2020-11-19)
Marek's disease (MD) is an oncogenic, lymphoproliferative, and highly contagious disease of chickens. Its etiologic agent is the alphaherpesvirus Marek's disease virus (MDV, Gallid alphaherpesvirus 2), and it is a chronic and ubiquitous problem for the poultry industry with significant
Joshua Phelan et al.
Talanta, 211, 120737-120737 (2020-02-20)
This is the first demonstration of the study of glycan protein turnover in living cells by FTIR with commercially available tetraacetylated N-Azidoacetyl-D-Mannosamine (Ac4ManNAz) label. The FTIR analysis has shown to be able to monitor the metabolism of glycans in living
Bernardo Saucedo et al.
Scientific reports, 9(1), 4453-4453 (2019-03-16)
Ranavirus is the second most common infectious cause of amphibian mortality. These viruses affect caudates, an order in which information regarding Ranavirus pathogenesis is scarce. In the Netherlands, two strains (CMTV-NL I and III) were suspected to possess distinct pathogenicity
Effect of estradiol on vitellogenesis and oocyte development of female swimming crab, Portunus trituberculatus
Liu M, et al.
Aquaculture (Amsterdam, Netherlands), 486, 240-245 (2018)
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L-15 Media Formulation

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