MilliporeSigma
All Photos(1)

Documents

W1878

SAFC

Williams′ Medium E

With sodium bicarbonate, without L-glutamine and phenol red, liquid, sterile-filtered, suitable for cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Williams’ E medium
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no

shipped in

ambient

storage temp.

2-8°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
W4128W0397M0450
SAFC

W1878

Williams′ Medium E

Sigma-Aldrich

W4128

Williams′ Medium E

SAFC

W0397

Williams′ Medium E

Sigma-Aldrich

M0450

Minimum Essential Medium Eagle

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

form

liquid

form

liquid

form

liquid

form

liquid

components

sodium pyruvate: 0.025 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L
L-glutamine: no

components

L-glutamine: no
phenol red: 0.0107 g/L
glucose: 2.0 g/L (Dextro)
NaHCO3: 2.2 g/L

components

NaHCO3: 2.2 g/L
phenol red: no
glucose: 2.0 g/L (Dextro)
sodium pyruvate: 0.025 g/L
L-glutamine: 0.292 g/L

components

glucose: 1.0 g/L
L-glutamine: 0.292 g/L
phenol red: 0.011 g/L
NaHCO3: 2.2 g/L

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

General description

Williams′ Medium E was developed by Williams and Gunn as a modification to Williams′ Medium D. The medium is formulated to support long-term cell culture of adult liver epithelial cells. It is a modified Minimum Essential Medium (MEM) with altered glucose and amino acid content.

Application

Williams′ Medium E has been used in the culturing of human hepatocellular carcinoma (HCC) cells and hepatic cells.

Reconstitution

Supplement with 0.292 g/L L-glutamine.

Other Notes

Phenol red has been shown to interfere with the growth of some cells at low or cloning densities. Use this version of William's E when working with stem cells or cells at low densities.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Dariusz Ratman et al.
Nucleic acids research, 44(22), 10539-10553 (2016-09-01)
Adaptation to fasting involves both Glucocorticoid Receptor (GRα) and Peroxisome Proliferator-Activated Receptor α (PPARα) activation. Given both receptors can physically interact we investigated the possibility of a genome-wide cross-talk between activated GR and PPARα, using ChIP- and RNA-seq in primary
Nicole A Kratochwil et al.
The AAPS journal, 19(2), 534-550 (2017-01-05)
Early prediction of human clearance is often challenging, in particular for the growing number of low-clearance compounds. Long-term in vitro models have been developed which enable sophisticated hepatic drug disposition studies and improved clearance predictions. Here, the cell line HepG2
Robert D Mitchell et al.
Journal of biochemical and molecular toxicology, 30(8), 375-395 (2016-04-20)
New paradigms for human health risk assessment of environmental chemicals emphasize the use of molecular methods and human-derived cell lines. In this study, we examined the effects of the insect repellent DEET (N,N-diethyl-m-toluamide) and the phenylpyrazole insecticide fipronil (fluocyanobenpyrazole) on
Balakrishnan Chakrapani Narmada et al.
Stem cells translational medicine, 6(3), 851-863 (2017-03-16)
Atherosclerosis underlies many cardiovascular and cerebrovascular diseases. Nutraceuticals are emerging as a therapeutic moiety for restoring vascular health. Unlike small-molecule drugs, the complexity of ingredients in nutraceuticals often confounds evaluation of their efficacy in preclinical evaluation. It is recognized that
Roger D Lawrie et al.
Pesticide biochemistry and physiology, 167, 104607-104607 (2020-06-13)
Human exposure to environmental chemicals both individually and in combination occurs frequently world-wide most often with unknown consequences. Use of molecular approaches to aide in the assessment of risk involved in chemical exposure is a growing field in toxicology. In

Protocols

Discover our collection of primary human hepatic Kupffer cells and protocol for thawing, plating, and growing Kupffer cells. Find the formulation for Kupffer culture maintenance media.

Discover our primary human hepatocytes and plating and maintenance media formulations. Explore our protocol for plateable primary hepatocyte cell culture.

Discover our protocol for thawing, plating, and forming hepatocyte spheroids from human spheroid-qualified plateable hepatocytes. Find the formulation for hepatocyte spheroid maintenance media.

Related Content

Explore our protocol for suspension primary hepatocyte cell culture. Discover our primary human hepatocytes and plating and maintenance media formulations.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service