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H8264

Sigma-Aldrich

Hanks′ Balanced Salt solution

Modified, with sodium bicarbonate, without phenol red, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
HBSS
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

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vibrant-m

H8264

Hanks′ Balanced Salt solution

vibrant-m

H6648

Hanks′ Balanced Salt solution

vibrant-m

H9394

Hanks′ Balanced Salt solution

vibrant-m

H9269

Hanks′ Balanced Salt solution

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

components

NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

400

General description

Hanks’ Balanced Salt solution (HBSS) is a buffer that mimics biological medium and stabilizes cells. It is useful in leucocyte labeling studies. HBSS is an effective preservative for periodontal ligament cells. HBSS lacks essential inorganic ions and is useful in starvation studies.

Application

Calcium and magnesium are included in this formulation and support cell adhesion. Used to minimize cell attachment or aggregation.


Hanks’ Balanced Salt solution has been used:

  • as a rinse solution for hepatocytes
  • for hippocampi dissection
  • in corrosion testing of dog-bone specimens
  • for starvation studies in breast cancer cell line
  • as a component of dissociation solution for dorsal root ganglion
  • as a chamber solution for the patch-clamp apparatus

Other Notes

Phenol red has been shown to interfere with the growth of some cells at cloning densities. Use this medium when working with stem cells or when growing cells at low densities.

supplement

Product No.
Description
Pricing

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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