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H9269

Sigma-Aldrich

Hanks′ Balanced Salt solution

With sodium bicarbonate, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
HBSS

Quality Level

sterility

sterile-filtered

form

liquid

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

components

NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

shipped in

ambient

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This Item
H8264H939455037C
vibrant-m

H9269

Hanks′ Balanced Salt solution

vibrant-m

H8264

Hanks′ Balanced Salt solution

vibrant-m

H9394

Hanks′ Balanced Salt solution

vibrant-m

55037C

Hanks′ Balanced Salt solution

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

form

liquid

form

liquid

form

liquid

form

liquid

components

NaHCO3: 0.35 g/L
phenol red: 0.011 g/L
glucose: 1.0 g/L (Dextro)

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: 0.011 g/L

components

NaHCO3: 350 mg/L
phenol red: no

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

Quality Level

400

Quality Level

400

Quality Level

400

Quality Level

500

General description

Formulation: click here.

Application

Hanks′ balanced salt solution has been used:
  • in starvation experiments for mammalian cells
  • to collect and maintain brain pieces for primary glial cell culture
  • to wash femoral tissue and bone marrow (BM) to culture mesenchymal stem cells

Biochem/physiol Actions

Balanced salt solution (BSS) in cell culture is multifaceted and has various functions. It serves as an irrigating, transporting, and diluting fluid while maintaining intra- and extracellular osmotic balance. BSS provides cells with water and certain bulk inorganic ions essential for normal cell metabolism. It combines with carbohydrates, such as glucose to act as the principal energy source for cell metabolism. BSS also provides a buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

The 96 Well 3D Spheroid BME Cell Invasion Assay utilizes 3D Culture Qualified 96 Well Spheroid Formation Plate alongside a specialized Spheroid Formation ECM to drive aggregation and/or spheroid formation of cells.

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