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H4641

Sigma-Aldrich

Hanks′ Balanced Salt Solution 10x

Without calcium chloride, magnesium sulfate and sodium bicarbonate, 10 ×, liquid, sterile-filtered, suitable for cell culture

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Synonym(s):
Hanks’ Balanced Salt solution, HBSS
NACRES:
NA.75

Quality Level

sterility

sterile-filtered

form

liquid

concentration

10 ×

technique(s)

cell culture | mammalian: suitable

components

phenol red: yes

shipped in

ambient

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This Item
H1641H6648H8264
Sigma-Aldrich

H4641

Hanks′ Balanced Salt Solution 10x

Sigma-Aldrich

H1641

Hanks′ Balanced Salt Solution 10x

Sigma-Aldrich

H6648

Hanks′ Balanced Salt solution

Sigma-Aldrich

H8264

Hanks′ Balanced Salt solution

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

sterility

sterile-filtered

form

liquid

form

liquid

form

liquid

form

liquid

components

phenol red: yes

components

-

components

NaHCO3: 0.35 g/L
glucose: 1.0 g/L (Dextro)
phenol red: no

components

phenol red: no
glucose: 1.0 g/L (Dextro)
NaHCO3: 0.35 g/L

shipped in

ambient

shipped in

-

shipped in

ambient

shipped in

ambient

concentration

10 ×

concentration

10 ×

concentration

-

concentration

-

General description

Balanced salt solution (BSS) is crucial in cell culture as it serves as an irrigating, transporting, and diluting agent to maintain the intra- and extracellular osmotic balance and also provides energy for cell metabolism. It provides water and bulk inorganic ions to the cells to maintain the normal cell metabolism. BSS plays a role in the buffering system to maintain the medium within the physiological pH range (7.2-7.6).

Application

Hanks′ Balanced Salt Solution 10x has been used:

  • as a diluting agent during the Chinese hamster ovary cell line detachment from growth medium
  • to wash the biopsy specimens of lamina propria lymphocytes (LPLs)
  • as a wash solution to wash the fish cell line during removal from growth medium

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate at 1X.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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