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H6136

SAFC

Hanks′ Balanced Salts

Without sodium bicarbonate, powder, suitable for cell culture

Pharma Manufacturing

Synonym(s):

Hanks′ Balanced Salt Solution

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About This Item

MDL number:
MFCD00217418
UNSPSC Code:
12352207
NACRES:
NA.75

Quality Level

500

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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General description

Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).

Application

Hanks′ Balanced Salt has been used:

  • in the preparation of stock solution for culturing mice primary hepatocytes
  • to measure the mineralized nodule formation in human osteoblast cultures
  • in the preparation of embryo buffer to collect zebrafish eggs

Quantity

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)

Certificates of Analysis (COA)

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Behavioral profiling of zebrafish embryos exposed to a panel of 60 water-soluble compounds
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Behavioural Brain Research, 228, 272-283 (2012)
Osteoblast response to porous titanium and biomimetic surface: In vitro analysis
Renata F, et al.
Materials Science and Engineering, C, 52, 194-203 (2015)
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Winged aphids are described as hosts of lesser quality for parasitoids because a part of their resources is used to produce wings and associated muscles during their development. Host lipid content is particularly important for parasitoid larvae as they lack
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Protocols

Powdered Media Preparation Instructions

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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