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H6136

SAFC

Hanks′ Balanced Salts

Without sodium bicarbonate, powder, suitable for cell culture

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MDL number:
NACRES:
NA.75

Quality Level

form

powder

technique(s)

cell culture | mammalian: suitable

storage temp.

2-8°C

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This Item
H1387H4891H1641
SAFC

H6136

Hanks′ Balanced Salts

Sigma-Aldrich

H1387

Hanks′ Balanced Salts

SAFC

H4891

Hanks′ Balanced Salts

Sigma-Aldrich

H1641

Hanks′ Balanced Salt Solution 10x

Quality Level

500

Quality Level

500

Quality Level

500

Quality Level

400

form

powder

form

powder

form

powder

form

liquid

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

-

HBSS, salt solutions

HBSS, salt solutions

HBSS, salt solutions

HBSS, salt solutions

General description

Balanced Salt solutions (BSS) are essential in cell culture as they serve as irrigating, transporting, and diluting agents to maintain the osmotic balance between intra-and extracellular spaces. They provide cells with water, inorganic ions, and energy for normal cell metabolism. BSS also provides a buffering system to maintain the medium within the optimum pH range (7.2-7.6).

Application

Hanks′ Balanced Salt has been used:

  • in the preparation of stock solution for culturing mice primary hepatocytes
  • to measure the mineralized nodule formation in human osteoblast cultures
  • in the preparation of embryo buffer to collect zebrafish eggs

Quantity

Formulated to contain 9.8 grams of powder per liter of medium.

Reconstitution

Supplement with 0.35 g/L sodium bicarbonate.

Other Notes

The pH is adjusted after addition of sodium bicarbonate.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type N95 (US)


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Protocols

Powdered media and salt mixtures are extremely hygroscopic and should be protected from atmospheric moisture.

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