Documents
H4034
HEPES
BioPerformance Certified, ≥99.5% (titration), suitable for cell culture
Recommended Products
grade
BioPerformance Certified
Quality Level
Assay
≥99.5% (titration)
form
crystalline powder
technique(s)
cell culture | mammalian: suitable
impurities
endotoxin and total aerobic microbial count, tested
useful pH range
6.8-8.2
pKa (25 °C)
7.5
cation traces
Fe: ≤5 ppm
absorption
≤0.05 at 260 in H2O at 0.1 M
≤0.05 at 280 in H2O at 0.1 M
application(s)
diagnostic assay manufacturing
foreign activity
DNase, RNase, NICKase, protease, none detected
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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Related Categories
1 of 4
This Item | H6147 | H7523 | H23830 |
|---|---|---|---|
| assay ≥99.5% (titration) | assay ≥99.5% (titration) | assay ≥99.5% (titration) | assay ≥99% (titration) |
| form crystalline powder | form powder | form powder | form crystalline |
| technique(s) cell culture | mammalian: suitable | technique(s) cell culture | embryo: suitable | technique(s) - | technique(s) - |
| impurities endotoxin and total aerobic microbial count, tested | impurities - | impurities Insoluble matter, passes filter test | impurities - |
| cation traces Fe: ≤5 ppm | cation traces - | cation traces Al: ≤0.0005%, As: ≤0.0001%, Ba: ≤0.0005%, Bi: ≤0.0005%, Ca: ≤0.001%, Cd: ≤0.0005%, Co: ≤0.0005%, Cr: ≤0.0005%, Cu: ≤0.0005%, Fe: ≤0.0005%, K: ≤0.005%, Li: ≤0.0005%, Mg: ≤0.0005%, Mn: ≤0.0005%, Mo: ≤0.0005%, Na: ≤0.005%, Ni: ≤0.0005%, Pb: ≤0.0005%, Sr: ≤0.0005%, Zn: ≤0.0005% | cation traces - |
General description
Application
- To supplement Dulbecco′s modified Eagle′s medium to culture and maintain cell lines
- As a component of platelet suspension buffer
- To supplement Hank′s basic salt solution, which is used to wash pancreatic tissue
- As a component of wash buffer and blocking buffer in the purification and quantification of protein with enzyme-linked immunosorbent (ELISA) assay
- For the adjustment and maintenance of pH of biological solutions
- As a component of Hank′s balanced salt solution (HBSS) and dissociation medium to study neuronal development
- For homogenization of tissue and in the preparation of cytosolic and nuclear extract from cells
- As a component of keratinocyte and fibroblast culture medium
Other Notes
also commonly purchased with this product
Storage Class Code
11 - Combustible Solids
WGK
WGK 1
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Personal Protective Equipment
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Protocols
This protocol describes a method for chemical cross-linking of proteins using formaldehyde. With the exception of zero-length cross-linkers, formaldehyde has the shortest cross-linking span (~2-3 Å) of any cross-linking reagent, thus making it an ideal tool for detecting specific protein-protein interactions with great confidence.
To determine the molecular weights of protein antigens, to study protein/protein interactions, to determine specific enzymatic activity, to monitor protein post-translational modifications and to determine the presence and quantity of proteins.
Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.
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