H7523
HEPES
BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)
Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
About This Item
Recommended Products
product line
BioXtra
Quality Level
assay
≥99.5% (titration)
form
powder
impurities
Insoluble matter, passes filter test
ign. residue (900°C)
≤0.1% (as SO4)
loss
≤1.0% loss on drying, 110°C
pH
5.0-6.5 (1 M in H2O)
useful pH range
6.8-8.2
pKa (25 °C)
7.5
solubility
H2O: 1 M, clear, colorless
anion traces
chloride (Cl-): ≤0.05%
cation traces
Al: ≤0.0005%
As: ≤0.0001%
Ba: ≤0.0005%
Bi: ≤0.0005%
Ca: ≤0.001%
Cd: ≤0.0005%
Co: ≤0.0005%
Cr: ≤0.0005%
Cu: ≤0.0005%
Fe: ≤0.0005%
K: ≤0.005%
Li: ≤0.0005%
Mg: ≤0.0005%
Mn: ≤0.0005%
Mo: ≤0.0005%
Na: ≤0.005%
Ni: ≤0.0005%
Pb: ≤0.0005%
Sr: ≤0.0005%
Zn: ≤0.0005%
absorption
≤0.035 at 280 in H2O at 1 M
≤0.045 at 260 in H2O at 1 M
application(s)
diagnostic assay manufacturing
SMILES string
OCCN1CCN(CC1)CCS(O)(=O)=O
InChI
1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)
InChI key
JKMHFZQWWAIEOD-UHFFFAOYSA-N
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General description
Application
- As a component of homogenisation buffer used in protein sample preparation
- To neutralise PRP (platelet-rich plasma) solution as part of PRP preparation
- As a component in 2X PBS buffer, which is used in phosphate calcium transfection in cell line preparation
- As a supplement in RPMI 1640 medium used to culture human umbilical vein endothelial cells (HUVEC) and in M199 complete medium for ex-vivo treatment of testicular tissue.
Other Notes
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Storage Class
13 - Non Combustible Solids
wgk_germany
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
Certificates of Analysis (COA)
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