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H23830

Sigma-Aldrich

HEPES

reagent grade, ≥99% (titration), crystalline

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Synonym(s):
4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
MDL number:

biological source

synthetic

Quality Level

grade

reagent grade

Assay

≥99% (titration)

form

crystalline

useful pH range

6.8-8.2

pKa (25 °C)

7.5

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
54457H3375H7523
HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

Essential Grade
HEPES BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

54457

HEPES

Premium Grade
HEPES ≥99.5% (titration)

Sigma-Aldrich

H3375

HEPES

-
HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

Premium Grade
assay

≥99% (titration)

assay

≥99.5% (T)

assay

≥99.5% (titration)

assay

≥99.5% (titration)

form

crystalline

form

powder

form

crystalline powder

form

powder

Quality Level

200

Quality Level

-

Quality Level

-

Quality Level

-

biological source

synthetic

biological source

-

biological source

-

biological source

-

useful pH range

6.8-8.2

useful pH range

6.8-8.2

useful pH range

6.8-8.2

useful pH range

6.8-8.2

General description

HEPES, or 4-(2-hydroxyethyl)piperazine-1-ethane-sulfonic acid, serves as a zwitterionic N-substituted aminosulfonic acid buffer with exceptional buffering capacity (pH 6.8-8.2, pKa 7.48 at 25°C). Recognized for versatility, it is extensively employed in cell biology, biochemical, and biological research. Its noteworthy feature lies in its neutrality towards metal ions, making it a preferred "non-coordinating buffer" suitable for solutions containing metals without affecting their activity. In cell biology studies, HEPES excels at maintaining stable physiological pH despite carbon dioxide fluctuations. It outperforms bicarbonate buffers commonly used in this context.

HEPES extends its utility as a preferred separator in creating pH gradients for isoelectric focusing, a technique in protein separation. Beyond these applications, it proves beneficial in immunoprecipitation, cell lysis, live cell imaging, establishing its indispensability across diverse research areas due to its versatility and minimal interaction with other molecules.

Application

HEPES has been used:
  • as a component in Danieau medium, which is used to prevent melanin pigment formation post gastrulation of zebra-fish embryo
  • as a component of Hanks′ solution used in the degradation testing of Iron
  • as a component of HEPES medium in the transfection of cell culture with plasmids
  • along with RPMI-1640, L-glutamine, FBS, Sodium pyruvate and β-mercaptoethanol for the culturing of rat insulinoma cells
  • as a supplement in TCM199 medium for washing fresh oocytes required for nuclear transfer studies

Features and Benefits

  • High purity product for biochemical and biological research
  • Highly soluble in water with a useful pH range of 6.8 - 8.2 and pKa of 7.5 at 25 °C

  • Broad buffer range between pH 6.8 to 8.2
  • Zwitterionic at biological pH
  • High solubility and stability
  • Low UV and visible light spectrum absorbance
  • Negligible metal ion binding
  • Suitable for cell culture, electrophoresis, in vitro methods, etc.

Storage Class Code

11 - Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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