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391340

Millipore

HEPES, Free Acid, Molecular Biology Grade

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

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Synonym(s):
HEPES, N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein:
883043
MDL number:

grade

Molecular Biology

Quality Level

Assay

≥99% (alkalimetric)

form

solid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 400 mg/mL
aqueous base: soluble

cation traces

heavy metals: ≤0.0005%

foreign activity

DNase, RNase, and protease, none detected

shipped in

ambient

storage temp.

15-25°C

SMILES string

OCCN1CCN(CC1)CCS(O)(=O)=O

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
54457H23830H6147
HEPES BioUltra, for molecular biology, ≥99.5% (T)

Sigma-Aldrich

54457

HEPES

Premium Grade
HEPES reagent grade, ≥99% (titration), crystalline

Sigma-Aldrich

H23830

HEPES

Essential Grade
HEPES BioXtra, suitable for mouse embryo cell culture, ≥99.5% (titration)

Sigma-Aldrich

H6147

HEPES

Premium Grade
assay

≥99% (alkalimetric)

assay

≥99.5% (T)

assay

≥99% (titration)

assay

≥99.5% (titration)

form

solid

form

powder

form

crystalline

form

powder

storage condition

OK to freeze

storage condition

-

storage condition

-

storage condition

-

color

white

color

-

color

-

color

-

solubility

water: 400 mg/mL, aqueous base: soluble

solubility

H2O: 1 M at 20 °C, clear, colorless

solubility

-

solubility

water: 500 mg/mL, clear, colorless

General description

A zwitterionice N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.

Warning

Toxicity: Standard Handling (A)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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25G
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705578-5MG-PW

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