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H0887

Sigma-Aldrich

HEPES solution

1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
7365-45-9
MDL number:
MFCD00006158
PubChem Substance ID:
24895414
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

300

product line

BioReagent
BioXtra

form

liquid

storage condition

(Tightly closed. Dry)

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

pH

7.0-7.6

useful pH range

6.8-8.2

storage temp.

2-8°C

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
H3537H7523H4034
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

H0887

HEPES solution

HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

Sigma-Aldrich

H3537

HEPES solution

HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

Sigma-Aldrich

H7523

HEPES

HEPES BioPerformance Certified, ≥99.5% (titration), suitable for cell culture

Sigma-Aldrich

H4034

HEPES

product line

BioReagent, BioXtra

product line

-

product line

BioXtra

product line

-

form

liquid

form

liquid

form

powder

form

crystalline powder

storage condition

(Tightly closed. Dry)

storage condition

-

storage condition

-

storage condition

-

concentration

1 M

concentration

1 M

concentration

-

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

cell culture | mammalian: suitable

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2 (pKa 7.55). It is typically used in cell culture at concentration between 5mM to 30 mM. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

HEPES has been used as a component of:
  • Hank′s balanced salt solution, Dulbecco′s modified eagle′s medium and no-glucose DMEM, which are used for the preparation of tissue slices
  • Buffers used in localized conductance measurements on the basolateral side of culture inserts to examine cation selectivity

Caution

Concentrations of HEPES over 40mM may be toxic to some cell types.

Preparation Note

Prepared from HEPES free acid and cell culture grade water.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

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