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H3537

Sigma-Aldrich

HEPES solution

BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

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Synonym(s):
N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)
CAS Number:
7365-45-9
MDL number:
MFCD00006158
PubChem Substance ID:
329815199
NACRES:
NA.25

grade

BioPerformance Certified

Quality Level

200

sterility

0.2 μm filtered

form

liquid

concentration

1 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
 DNase, RNase, Protease, Nickase, free
 endotoxin, tested

pH

5.0-6.0

useful pH range

6.8-8.2

cation traces

Fe: <5 ppm
heavy metals (as Pb): <5 ppm

suitability

suitable for molecular biology

application(s)

diagnostic assay manufacturing

SMILES string

OCCN1CCN(CCS(=O)(O)=O)CC1

InChI

1S/C8H18N2O4S/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14/h11H,1-8H2,(H,12,13,14)

InChI key

JKMHFZQWWAIEOD-UHFFFAOYSA-N

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This Item
H088783264H7523
HEPES solution BioPerformance Certified, 1&#160;M, suitable for cell culture, 0.2 &#956;m filtered

Sigma-Aldrich

H3537

HEPES solution

HEPES solution 1&#160;M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

Sigma-Aldrich

H0887

HEPES solution

HEPES buffer solution 1&#160;M in H2O

Sigma-Aldrich

83264

HEPES buffer solution

HEPES BioXtra, pH 5.0-6.5 (1&#160;M in H2O), &#8805;99.5% (titration)

Sigma-Aldrich

H7523

HEPES

sterility

0.2 μm filtered

sterility

sterile-filtered

sterility

-

sterility

-

form

liquid

form

liquid

form

liquid

form

powder

concentration

1 M

concentration

1 M

concentration

1 M in H2O, 238 g/L HEPES in H2O

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

-

technique(s)

-

impurities

Bioburden, tested, DNase, RNase, Protease, Nickase, free, endotoxin, tested

impurities

endotoxin, tested

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

Insoluble matter, passes filter test

General description

HEPES has been described as one of the best all-purpose buffers available for biological research. At biological pH, the molecule is zwitterionic, and is effective as a buffer at pH 6.8 to 8.2. HEPES has been used in a wide variety of applications, including tissue culture. It is commonly used to buffer cell culture media in air. HEPES finds its usage in in vitro experiments on Mg.

Application

RNAse HEPES has been used:
  • To supplement Dulbecco′smodified Eagle′smedium for maintenance of cell line and RPMI medium to wash rat islets
  • To recover purified ribonucleotide
  • As a component of HEPES/KOH buffer and adenylation buffer for small RNA isolation and sequencing
  • As a component in buffers used for nuclear extract preparation and also to supplement RPMI-1640 medium for maintenance of islets

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

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Timing of CRISPR/Cas9-related mRNA microinjection after activation as an important factor affecting genome editing efficiency in porcine oocytes.
Sato M, et al.
Theriogenology, 108, 29-38 (2018)
A Small RNA Isolation and Sequencing Protocol and Its Application to Assay CRISPR RNA Biogenesis in Bacteria.
Silas S, et al.
Bio-protocol, 8(4) (2018)
Masahiro Sato et al.
Theriogenology, 108, 29-38 (2017-12-02)
Recently, successful one-step genome editing by microinjection of CRISPR/Cas9-related mRNA components into the porcine zygote has been described. Given the relatively long gestational period and the high cost of housing swine, the establishment of an effective microinjection-based porcine genome editing
Expression of an uncleavable N-terminal RasGAP fragment in insulin-secreting cells increases their resistance toward apoptotic stimuli without affecting their glucose-induced insulin secretion.
Yang JY, et al.
The Journal of Biological Chemistry, 280(38), 32835-32842 (2005)
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International journal of molecular sciences, 16(8), 17838-17856 (2015-08-08)
Some reports demonstrated successful genome editing in pigs by one-step zygote microinjection of mRNA of CRISPR/Cas9-related components. Given the relatively long gestation periods and the high cost of housing, the establishment of a single blastocyst-based assay for rapid optimization of
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