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SRE0065

Sigma-Aldrich

HEPES solution, 1M

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Synonym(s):
HEPES Buffer, N-(2-Hydroxyethyl)piperazineN′-(2-ethanesulfonic acid)

form

liquid

Quality Level

concentration

1 M

color

colorless

pH

7.2-7.5 (25 °C)

application(s)

diagnostic assay manufacturing
hematology
histology

storage temp.

room temp

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SRE0065

HEPES solution, 1M

-
MES solution suitable for, BioPerformance Certified, 1 M, suitable for cell culture

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Premium Grade
HEPES solution BioPerformance Certified, 1 M, suitable for cell culture, 0.2 μm filtered

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HEPES solution

Premium Grade
HEPES solution 1 M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

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H0887

HEPES solution

Essential+ Grade
concentration

1 M

concentration

1 M

concentration

1 M

concentration

1 M

color

colorless

color

-

color

-

color

-

pH

7.2-7.5 (25 °C)

pH

-

pH

5.0-6.0

pH

7.0-7.6

application(s)

diagnostic assay manufacturing
hematology
histology

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

-

storage temp.

room temp

storage temp.

room temp

storage temp.

-

storage temp.

2-8°C

Application

HEPES buffer preserves a normal physiological pH environment and is commonly used as a buffer in cell culture applications. HEPES buffer provides no nutritional benefit to cells, but adds extra buffering capacity to the cell culture particularly during experiments performed outside the carbon dioxide incubator.
This 1 M stock solution contains HEPES adjusted to pH 7.35 with sodium hydroxide. Add the concentrated buffer to the cell culture medium to reach the desired concentration (typically 10–25 mM).

Notes: If precipitation is present in the concentrated buffer, gently warm the bottle to 37 °C and mix until completely dissolved prior to dilution.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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