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H3662

Sigma-Aldrich

HEPES sodium salt solution

1M, BioReagent, suitable for cell culture

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Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

sterility

sterile-filtered (0.2 μm filtered)

product line

BioReagent

form

solution

concentration

0.90-1.10 M

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested
DNase, RNase. Protease, Nickase, free
endotoxin, tested
<5 ppm Heavy metals (as Pb)

cation traces

Fe: <5 ppm

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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HEPES sodium salt solution 1M, BioReagent, suitable for cell culture

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HEPES solution 1&#160;M, pH 7.0-7.6, sterile-filtered, BioReagent, suitable for cell culture

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HEPES solution

Essential+ Grade
product line

BioReagent

product line

-

product line

BioReagent, BioXtra

product line

BioReagent, BioXtra

form

solution

form

powder

form

liquid

form

liquid

concentration

0.90-1.10 M

concentration

-

concentration

1 M

concentration

1 M

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

Bioburden, tested, DNase, RNase. Protease, Nickase, free, endotoxin, tested, <5 ppm Heavy metals (as Pb)

impurities

endotoxin and total aerobic microbial count, tested

impurities

endotoxin, tested

impurities

endotoxin, tested

General description

HEPES is a general-purpose zwitterionic buffer which does not bind magnesium, calcium, manganese(II) or copper(II) ions. HEPES has been classified as Good′s buffer by Dr. Norman Good and his colleagues in 1966. It is zwitterionic at biological pH and is most effective in the pH range of pH 6.8 to 8.2.

Application

HEPES sodium salt solution is suitable for use in cell culture studies. It may be used in the preparation of buffer solution of HEPES. It may be used as buffer during phosphorylation assays in permeabilized fibroblasts. It may be used to compose a complex dialysis buffer, used in the determination of free thyroxin in serum by radioimmunoassay.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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