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H7006

Sigma-Aldrich

HEPES sodium salt

≥99.0% (titration)

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Synonym(s):
Sodium 2-(4-(2-hydroxyethyl)piperazin-1-yl)ethanesulfonate, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid) sodium salt
Empirical Formula (Hill Notation):
C8H17N2NaO4S
CAS Number:
Molecular Weight:
260.29
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

Assay

≥99.0% (titration)

form

powder

pH

10-12 (1 M in water)

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 0.1 M, clear, colorless

application(s)

diagnostic assay manufacturing

storage temp.

room temp

SMILES string

[Na+].OCCN1CCN(CC1)CCS([O-])(=O)=O

InChI

1S/C8H18N2O4S.Na/c11-7-5-9-1-3-10(4-2-9)6-8-15(12,13)14;/h11H,1-8H2,(H,12,13,14);/q;+1/p-1

InChI key

RDZTWEVXRGYCFV-UHFFFAOYSA-M

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This Item
H3784RES6007H-A7H7637
HEPES sodium salt ≥99.0% (titration)

Sigma-Aldrich

H7006

HEPES sodium salt

-
HEPES sodium salt BioPerformance Certified, suitable for cell culture, ≥99.0%

Sigma-Aldrich

H3784

HEPES sodium salt

Premium Grade
HEPES sodium salt

SAFC

RES6007H-A7

HEPES sodium salt

-
HEPES hemisodium salt ≥99% (titration)

Sigma-Aldrich

H7637

HEPES hemisodium salt

-
form

powder

form

powder

form

powder

form

powder

pH

10-12 (1 M in water)

pH

10-12 (1 M in water)

pH

-

pH

7.4-7.7 (25 °C, 0.1 M in water)

solubility

water: 0.1 M, clear, colorless

solubility

water: 0.1 M, clear, colorless

solubility

-

solubility

water: soluble 0.5 g/mL

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

storage temp.

room temp

storage temp.

-

storage temp.

-

storage temp.

room temp

General description

HEPES salt, or 4-(2-hydroxyethyl)piperazine-1-ethanesulfonic acid sodium salt, is a zwitterionic biological buffer that is widely used in biological and biochemical research. It has a buffering range of pH 6.8 to 8.2, making it ideal for a variety of research applications. HEPES is also a popular choice for buffering cell culture media, offering advantages such as lower cell toxicity in comparison to alternatives like Tris and phosphate.

Widely utilized in cell biology and biochemical research, HEPES proves its versatility in protein purification and extraction protocols, owing to its stability across a broad pH range and minimal metal ion binding. Additionally, HEPES proves valuable in various biological and biochemical processes, including immunoprecipitation, cell lysis, and live cell imaging, making it a versatile tool in diverse research applications.

Application

HEPES buffer finds application in:
  • Most studies with metal ions
  • Substitute for Tris and phosphate in studies with metal ions
  • Grinding buffer in plant studies
  • Running buffer in gel electrophoresis
  • Buffer in electroporation studies
  • Bradford or bicinchoninic acid (BCA) assays
  • Extraction buffer to prevent damage of proteins in red blood cells

It has been used:
  • as a buffer component in the preparation of a low sucrose buffer
  • as a buffer component in a modified Hanks′ solution, a pseudo-physiological solutions

Features and Benefits

  • Tested to confirm low levels of heavy metal contamination, ensuring suitability for various applications
  • Highly soluble in water
  • Negligible metal ion binding
  • Less toxic to cells than other buffers such as Tris and phosphate
  • Stable in a wide pH range

Physical form

Lyophilized, spray dried, or crystallized material.

Other Notes

Easily compare specifications for HEPES sodium salt products with the HEPES sodium salt specification table.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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