Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

391338

Millipore

HEPES

≥99% dry basis (titration), crystalline powder, amniosulfonic acid buffer

Synonym(s):

HEPES, N-2-Hydroxyethylpiperazine-Nʹ-2-ethanesulfonic Acid, 4-(2-Hydroxyethyl)piperazine-1-ethanesulfonic acid, N-(2-Hydroxyethyl)piperazine-N′-(2-ethanesulfonic acid)

Sign Into View Organizational & Contract Pricing

Select a Size

25 G
$80.40
100 G
$114.00
500 G
$431.00
1 KG
$696.00
5 KG
$2,670.00

$80.40


Estimated to ship onMarch 22, 2025


Request a Bulk Order

Select a Size

Change View
25 G
$80.40
100 G
$114.00
500 G
$431.00
1 KG
$696.00
5 KG
$2,670.00

About This Item

Empirical Formula (Hill Notation):
C8H18N2O4S
CAS Number:
Molecular Weight:
238.30
Beilstein/REAXYS Number:
883043
MDL number:
UNSPSC Code:
12352106
NACRES:
NA.25

$80.40


Estimated to ship onMarch 22, 2025


Request a Bulk Order

Product Name

HEPES, ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

Quality Level

assay

≥99% dry basis (titration)

form

crystalline powder

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze

color

white

useful pH range

6.8-8.2

pKa (25 °C)

7.5

solubility

water: 1 M

Looking for similar products? Visit Product Comparison Guide

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
H7523H23830PHR1428
HEPES ULTROL® Grade, ≥99% dry basis (titration), zwitterionic N-substituted aminosulfonic acid buffer

391338

HEPES

HEPES BioXtra, pH 5.0-6.5 (1 M in H2O), ≥99.5% (titration)

H7523

HEPES

HEPES reagent grade, ≥99% (titration), crystalline

H23830

HEPES

HEPES Pharmaceutical Secondary Standard; Certified Reference Material

PHR1428

HEPES

Quality Level

100

Quality Level

400

Quality Level

200

Quality Level

300

form

crystalline powder

form

powder

form

crystalline

form

-

storage temp.

15-25°C

storage temp.

-

storage temp.

room temp

storage temp.

2-30°C

storage condition

OK to freeze

storage condition

-

storage condition

(Tightly closed. Dry)

storage condition

-

solubility

water: 1 M

solubility

H2O: 1 M, clear, colorless

solubility

water: 1 M, clear, colorless

solubility

-

General description

A zwitterionic N-substituted aminosulfonic acid buffer. HEPES has its maximum buffering capacity very near the pH of mammalian tissue fluids (pKa of 7.31 at 37°C). Exhibits no metal binding and has a small temperature dependent ΔpKa of -0.014/°C.
A zwitterionic N-substituted aminosulfonic acid buffer. Useful in the pH 6.8-8.2 range. Has a pKa of 7.48 at 25°C.
Absorbance (1.0 M, H2O, 260 nm):≤0.05.

Absorbance (1.0 M, H2O, 280 nm): ≤0.04

Warning

Toxicity: Irritant (B)

Reconstitution

Following reconstitution, store at room temperature (20°C). Stock solutions are stable for up to 6 months at 20°C.

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany
ULTROL is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


  • Certificates of Analysis (COA)

    Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

    Already Own This Product?

    Find documentation for the products that you have recently purchased in the Document Library.

    Visit the Document Library

    Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

    Contact Technical Service