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93283

Sigma-Aldrich

Tris-EDTA buffer solution

BioUltra, for molecular biology, pH 8.0

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Synonym(s):
TE buffer, TE buffer solution
MDL number:
eCl@ss:
32129211
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

product line

BioUltra

form

liquid

impurities

DNases, none detected
RNases, none detected
insoluble matter, passes filter test
phosphatases, none detected
proteases, none detected

pH

8.0±0.2 (25 °C)

cation traces

Al: ≤1 mg/kg
Ba: ≤1 mg/kg
Bi: ≤1 mg/kg
Ca: ≤5 mg/kg
Cd: ≤1 mg/kg
Co: ≤1 mg/kg
Cr: ≤1 mg/kg
Cu: ≤1 mg/kg
Fe: ≤1 mg/kg
K: ≤20 mg/kg
Li: ≤1 mg/kg
Mg: ≤1 mg/kg
Mn: ≤1 mg/kg
Mo: ≤1 mg/kg
Ni: ≤1 mg/kg
Pb: ≤1 mg/kg
Sr: ≤1 mg/kg
Zn: ≤1 mg/kg

λ

neat

UV absorption

λ: 260 nm Amax: 0.04
λ: 280 nm Amax: 0.01

application(s)

sample preparation

SMILES string

NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2

InChI key

VLEIUWBSEKKKFX-UHFFFAOYSA-N

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This Item
85810T9650T9285
Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0

Sigma-Aldrich

93283

Tris-EDTA buffer solution

Premium Grade
Sigma-Aldrich

Sigma-Aldrich

85810

STE buffer solution

Premium Grade
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

Sigma-Aldrich

T9650

Tris Acetate-EDTA buffer

Essential+ Grade
product line

BioUltra

product line

BioUltra

product line

BioReagent

product line

-

form

liquid

form

liquid

form

solution

form

liquid

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

DNases, none detected, RNases, none detected, insoluble matter, passes filter test, phosphatases, none detected, proteases, none detected

impurities

-

impurities

-

pH

8.0±0.2 (25 °C)

pH

7.8±0.2 (25 °C)

pH

-

pH

7.8-8.2 (1 × in water)

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

Al: ≤1 mg/kg, Ba: ≤1 mg/kg, Bi: ≤1 mg/kg, Ca: ≤5 mg/kg, Cd: ≤1 mg/kg, Co: ≤1 mg/kg, Cr: ≤1 mg/kg, Cu: ≤1 mg/kg, Fe: ≤1 mg/kg, K: ≤20 mg/kg, Li: ≤1 mg/kg, Mg: ≤1 mg/kg, Mn: ≤1 mg/kg, Mo: ≤1 mg/kg, Ni: ≤1 mg/kg, Pb: ≤1 mg/kg, Sr: ≤1 mg/kg, Zn: ≤1 mg/kg

cation traces

-

cation traces

-

General description

Tris EDTA is a biological buffer in research. It is a combination of Tris base and EDTA, a metal chelator. Tris provides buffering capacity, maintaining a stable pH in biological samples, while EDTA helps prevent metal ion interference by chelating divalent metal ions. Tris EDTA is often used in molecular biology techniques, such as DNA and RNA sample preparation, electrophoresis, and enzymatic reactions. It helps maintain the stability and integrity of nucleic acids by inhibiting the activity of nucleases and metal-dependent enzymes.

Application

TE buffer is useful for general extraction, suspension, washing, and storage buffer and in the preparation of DNA or RNA. Commonly used for DNA sequencing, restriction enzyme digestion; ligation and polymerase chain reaction (PCR) applications.

Features and Benefits

  • BioUltra grade - suitable for many molecular biology applications
  • DNase, RNase, phosphatase, and protease-free
  • High purity and free of trace metals

Storage Class Code

12 - Non Combustible Liquids

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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