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Sigma-Aldrich

TE Buffer, 100X, Molecular Biology Grade

A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.

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Synonym(s):
Tris-EDTA Buffer

grade

Molecular Biology

form

liquid

manufacturer/tradename

Calbiochem®

storage condition

OK to freeze
desiccated

foreign activity

DNase, RNase, protease, none detected

shipped in

ambient

storage temp.

15-25°C

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This Item
574795574797T9285
vibrant-m

T9285

Tris-EDTA buffer solution

form

liquid

form

liquid

form

liquid

form

liquid

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

15-25°C

storage temp.

room temp

storage condition

OK to freeze, desiccated

storage condition

OK to freeze

storage condition

OK to freeze, desiccated

storage condition

-

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, protease, none detected

foreign activity

DNase, RNase, none detected

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

Calbiochem®

manufacturer/tradename

-

General description

A 100X concentrate that, when diluted to 1X, contains 10 mM Tris, 1 mM EDTA, pH ~8.0.
Tris-ethylenediaminetetraacetic (TE) buffer is a common buffer used in molecular biology to maintain the integrity of nucleic acid during handling and storage.

Application

TE Buffer, 100X, Molecular Biology Grade – Calbiochem has been used to dilute DNA stock solutions. It has also been used to dilute thymidylate synthase (thyA) culture for colony polymerase chain reaction (PCR).

Warning

Toxicity: Irritant (B)

Legal Information

CALBIOCHEM is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1


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Victor Chen et al.
Applied and environmental microbiology, 87(18), e0084421-e0084421 (2021-07-08)
Enterococcus faecium is a ubiquitous Gram-positive bacterium that has been recovered from the environment, food, and microbiota of mammals. Commensal strains of E. faecium can confer beneficial effects on host physiology and immunity, but antibiotic usage has afforded antibiotic-resistant and
Ping Song et al.
Nature biomedical engineering, 5(7), 690-701 (2021-05-05)
DNA sequence variants with allele fractions below 1% are difficult to detect and quantify by sequencing owing to intrinsic errors in sequencing-by-synthesis methods. Although molecular-identifier barcodes can detect mutations with a variant-allele frequency (VAF) as low as 0.1% using next-generation
Jason W Johnston et al.
Plant physiology and biochemistry : PPB, 43(9), 844-853 (2005-11-18)
HPLC analysis of nucleosides is important for determining total DNA methylation in plants and can be used to help characterise epigenetic changes during stress, growth and development. This is of particular interest for in vitro plant cultures as they are

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