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T9650

Sigma-Aldrich

Tris Acetate-EDTA buffer

10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

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Synonym(s):
TAE buffer
MDL number:
PubChem Substance ID:
NACRES:
NA.25

grade

for molecular biology

Quality Level

sterility

non-sterile; 0.2 μm filtered

product line

BioReagent

form

solution

suitability

suitable for gel electrophoresis (after dilution to working concentration)

foreign activity

Protease, none detected
RNAse, none detected

SMILES string

CC(O)=O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.C2H4O2/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;1-2(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;1H3,(H,3,4)

InChI key

HGEVZDLYZYVYHD-UHFFFAOYSA-N

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This Item
T828093283T6025
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

T9650

Tris Acetate-EDTA buffer

Essential+ Grade
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

T8280

Tris Acetate-EDTA buffer

Essential+ Grade
Tris-EDTA buffer solution BioUltra, for molecular biology, pH 8.0

93283

Tris-EDTA buffer solution

Premium Grade
Tris Acetate-EDTA buffer BioReagent, suitable for electrophoresis

T6025

Tris Acetate-EDTA buffer

Essential+ Grade
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

product line

BioReagent

product line

BioReagent

product line

BioUltra

product line

BioReagent

form

solution

form

powder blend

form

liquid

form

working solution

sterility

non-sterile; 0.2 μm filtered

sterility

-

sterility

-

sterility

0.2 μm filtered

foreign activity

Protease, none detected, RNAse, none detected

foreign activity

-

foreign activity

-

foreign activity

-

Application

Tris Acetate-EDTA buffer has been used for the preparation of agarose gel during DNA agarose gel electrophoresis.
TAE running buffer is the most commonly used buffer for DNA agarose gel electrophoresis but is also used for non-denaturing RNA agarose gel electrophoresis. Double-stranded DNA tends to run faster in TAE than in other buffers but can also become exhausted during extended electrophoresis. Buffer circulation or buffer replacement during extended electrophoresis can remedy the lower buffering capacity. Dilution of the concentrated TAE buffer produces a 1× TAE buffer with 40 mM Tris-acetate and 1 mM EDTA, pH 8.3. The 1× TAE buffer is used both in the agarose gel and as a running buffer. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Other Notes

0.4 M Tris acetate, pH approx. 8.3, containing 0.01 M EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (T6066) and Molecular Biology Reagent EDTA disodium salt (E5134). Solutions also contain acetic acid (A6283); powdered blend contains Trizma acetate (T1258).
Solution prepared with 18 megohm water

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

STOT RE 2 Inhalation

target_organs

Respiratory Tract

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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Protocols

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

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