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T9525

Sigma-Aldrich

Tris-Borate-EDTA buffer

working solution

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Synonym(s):
TBE buffer
MDL number:
PubChem Substance ID:
NACRES:
NA.25

sterility

0.2 μm filtered

Quality Level

form

working solution

technique(s)

electrophoresis: suitable

impurities

DNase, RNase, Protease, none detected

suitability

suitable for gel electrophoresis (after dilution to working concentration)

application(s)

diagnostic assay manufacturing

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

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This Item
T9650T8280T7527
Tris-Borate-EDTA buffer working solution

Sigma-Aldrich

T9525

Tris-Borate-EDTA buffer

-
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, non-sterile; 0.2 μm filtered

Sigma-Aldrich

T9650

Tris Acetate-EDTA buffer

Essential+ Grade
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T8280

Tris Acetate-EDTA buffer

Essential+ Grade
Tris-Borate-EDTA buffer BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

Sigma-Aldrich

T7527

Tris-Borate-EDTA buffer

Essential+ Grade
form

working solution

form

solution

form

powder blend

form

powder blend

technique(s)

electrophoresis: suitable

technique(s)

-

technique(s)

-

technique(s)

-

impurities

DNase, RNase, Protease, none detected

impurities

-

impurities

DNase and RNase, none detected

impurities

DNase and RNase, none detected

suitability

suitable for gel electrophoresis (after dilution to working concentration)

suitability

suitable for gel electrophoresis (after dilution to working concentration)

suitability

-

suitability

-

application(s)

diagnostic assay manufacturing

application(s)

-

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

Application

TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Supplied in dispenser with a spigot.

Other Notes

89 mM Tris borate, pH approx. 8.3, containing 2 mM EDTA.

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).
Solution prepared with 18 megohm water

Pictograms

Health hazard

Signal Word

Danger

Hazard Statements

Hazard Classifications

Repr. 1B

Storage Class Code

6.1D - Non-combustible, acute toxic Cat.3 / toxic hazardous materials or hazardous materials causing chronic effects

WGK

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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