MilliporeSigma
All Photos(1)

Documents

T3913

Sigma-Aldrich

Tris-Borate-EDTA buffer

5× concentrate, powder blend

Sign Into View Organizational & Contract Pricing

Synonym(s):
TBE buffer
MDL number:
PubChem Substance ID:
NACRES:
NA.25

Quality Level

form

powder blend

technique(s)

electrophoresis: suitable

impurities

DNase, RNase and NICKase, none detected

pH

8.1-8.5 (25 °C, 5 ×)

solubility

water: 85.1 g/L, clear, colorless

suitability

suitable for gel electrophoresis (after dilution to working concentration)

application(s)

diagnostic assay manufacturing

SMILES string

OB(O)O.NC(CO)(CO)CO.OC(=O)CN(CCN(CC(O)=O)CC(O)=O)CC(O)=O

InChI

1S/C10H16N2O8.C4H11NO3.BH3O3/c13-7(14)3-11(4-8(15)16)1-2-12(5-9(17)18)6-10(19)20;5-4(1-6,2-7)3-8;2-1(3)4/h1-6H2,(H,13,14)(H,15,16)(H,17,18)(H,19,20);6-8H,1-3,5H2;2-4H

InChI key

OSBLTNPMIGYQGY-UHFFFAOYSA-N

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
T7527T8280T4038
Tris-Borate-EDTA buffer 5× concentrate, powder blend

T3913

Tris-Borate-EDTA buffer

-
Tris-Borate-EDTA buffer BioReagent, for molecular biology, 5x concentrate, DNase and RNase, none detected, powder blend, suitable for electrophoresis

T7527

Tris-Borate-EDTA buffer

Essential+ Grade
Tris Acetate-EDTA buffer 10× concentrate, BioReagent, for molecular biology, DNase and RNase, none detected, powder blend, suitable for electrophoresis

T8280

Tris Acetate-EDTA buffer

Essential+ Grade
Tris Acetate-EDTA buffer DNase and RNase, none detected, BioReagent, suitable for electrophoresis, 10× concentrate

T4038

Tris Acetate-EDTA buffer

Essential+ Grade
Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

application(s)

diagnostic assay manufacturing

form

powder blend

form

powder blend

form

powder blend

form

powder blend

pH

8.1-8.5 (25 °C, 5 ×)

pH

8.1-8.5 (5 ×)

pH

8.1-8.5

pH

8.1-8.5

solubility

water: 85.1 g/L, clear, colorless

solubility

water: 85.1 g/L, clear, colorless

solubility

-

solubility

water: 64.2 g/L, clear, colorless

Application

Tris-Borate-EDTA buffer is suitable for use in gel electrophoresis.
It may be employed for the following studies:
  • As buffer system for the evaluation of molecular weights of glycoproteins of ovalbumin, avidin and fetuin by sodium dodecyl sulfate-pore gradient electrophoresis.
  • For the hemoglobin electrophoresis in starch gel.
  • Preparation of buffer concentration gradient gel for the rapid determination of DNA sequence.
TBE running buffer is the most commonly used buffer for DNA and RNA polyacrylamide gel electrophoresis. TBE is used with non-denaturing or denaturing (7 M urea) gels. It is also routinely used for DNA automated sequencing gel. TBE can also be used for agarose gels but is not recommended for preparative gels for recovery of nucleic acids.
Dilution of the TBE stock concentrates to a 1× TBE running buffer results in a buffer containing 89 mM Tris-borate and 2 mM EDTA, pH 8.3. The 5× or 10× stocks may also be added to an acrylamide/bis-acrylamide stock solution for making the PAGE gel. Applied voltages of less than 5 V/cm (distance between the electrodes of the unit) are recommended for maximum resolution.

Packaging

Packaged in pouches

Preparation Note

Prepared with Biotechnology Performance Certified Trizma base (Product Code T6066) and Molecular Biology Reagents boric acid (Product Code B6768) and EDTA disodium salt (Product Code E5134).

Reconstitution

Produces a 5× concentrate (0.445 M Tris-borate, 10 mM EDTA, pH 8.3) after dissolving with the indicated amount of water. A suitable container must be supplied.

pictograms

Health hazard

signalword

Danger

hcodes

Hazard Classifications

Repr. 1B

Storage Class

6.1C - Combustible, acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, type P2 (EN 143) respirator cartridges


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Customers Also Viewed

Slide 1 of 4

1 of 4

Glycoprotein molecular-weight estimation using sodium dodecyl sulfate-pore gradient electrophoresis: comparison of tris-glycine and tris-borate-EDTA buffer systems.
J F Poduslo
Analytical biochemistry, 114(1), 131-139 (1981-06-01)
M D Biggin et al.
Proceedings of the National Academy of Sciences of the United States of America, 80(13), 3963-3965 (1983-07-01)
Two methods for increasing the length of DNA sequence data that can be read off a polyacrylamide gel are described. We have developed a rapid way to pour a buffer concentration gradient gel that, by altering the vertical band separation
THE AMINO ACID COMPOSITION OF HEMOGLOBIN. V. THE PREPARATION OF PURIFIED HEMOGLOBIN FRACTIONS BY CHROMATOGRAPHY ON CELLULOSE EXCHANGERS AND THEIR IDENTIFICATION BY STARCH GEL ELECTROPHORESIS USING TRIS-BORATE-EDTA BUFFER.
A I CHERNOFF et al.
Blood, 25, 646-661 (1965-05-01)
Xiang Li et al.
Nucleic acids research, 47(10), 5074-5085 (2019-06-05)
In microorganisms, a number of metalloproteins including PerR are found to regulate gene expression in response to environmental reactive oxygen species (ROS) changes. However, discovery of similar regulatory mechanisms remains elusive within mammalian cells. As an antioxidant metalloenzyme that maintains
Josep Balart et al.
Radiation oncology (London, England), 6, 6-6 (2011-01-18)
Radiation-induced DNA double-strand break (DSB) repair can be tested by using pulsed-field gel electrophoresis (PFGE) in agarose-encapsulated cells. However, previous studies have reported that this assay is impaired by the spontaneous DNA breakage in this medium. We investigated the mechanisms

Protocols

TAE and TBE are both used as running buffers for nucleic acid electrophoresis but have some important differences. Review our recipes and video to give your application the best chance of success.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service