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L8384

Sigma-Aldrich

Linoleic Acid-Albumin from bovine serum albumin

γ-irradiated, lyophilized powder, BioReagent, suitable for cell culture

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About This Item

UNSPSC Code:
12352202
NACRES:
NA.75

biological source

bovine plasma

Quality Level

200

description

Contains 1.1-2.3 moles linoleic acid per mole BSA

sterility

γ-irradiated

product line

BioReagent

form

lyophilized powder

concentration

>0.5% (linolenic acid)

technique(s)

cell culture | mammalian: suitable

impurities

≤50 EU/mg endotoxin

solubility

water: 10 mg/mL, clear to hazy, colorless to light yellow

storage temp.

2-8°C

Related Categories

General description

Linoleic acid is an essential and highly consumed polyunsaturated fatty acid (PUFA). Albumin is a globular protein. Bovine serum albumin (BSA) is composed of a single polypeptide chain and cross-linked cysteine disulfide bridges. BSA is heart-shaped and is made up of three homologous domains I-III and each domain has two subdomains A and B.

Application

Linoleic Acid-Albumin from bovine serum albumin has been used: in cell culture and to study its effects on the human colonic adenocarcinoma cell line. It has also been used in the in vitro culture (IVC) of cow embryos/zygotes. The binding of linoleic acid to BSA improves its stability and solubility in culture media.

Biochem/physiol Actions

Linoleic acid plays a role in maintaining the transdermal water barrier in epidermis. Serum albumin constitutes about 52-62% of the total water-soluble fraction proteins in the blood plasma.

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Description
Pricing

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable

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Certificates of Analysis (COA)

Lot/Batch Number
SLCM4492
SLCD4647
SLBZ0308
SLBQ5313V
SLBF1632V

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Takemasa Hidaka et al.
Theriogenology, 113, 176-182 (2018-03-20)
Variations in embryo production between individual oocyte donors represent a serious problem in cattle production, when implementing ovum pick-up (OPU) and in vitro maturation (IVM) of oocytes. However, the precise cause of this problem is unknown. Here, we aimed to investigate

Articles

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

Frequently Asked Questions About Gamma-Irradiated FBS (Serum) and Other Products for Cell Culture

Find answers to questions (FAQs) about fetal bovine serum (FBS) that is gamma-irradiated by the SER-TAIN™ process. Request a sample of FBS at Sigma-Aldrich.com.

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