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L9530

Sigma-Aldrich

Linoleic Acid-Albumin from bovine serum albumin

liquid, sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
Linoleic Acid-Albumin from BSA
NACRES:
NA.75

description

Contains 2 moles linoleic acid per mole BSA

Quality Level

300

sterility

sterile-filtered

product line

BioReagent

form

liquid

concentration

100 mg/mL BSA in DPBS
90-120 mg/mL protein (biuret)

technique(s)

cell culture | mammalian: suitable

impurities

≤400 EU/mL endotoxin

storage temp.

2-8°C

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This Item
A6003O3008L8384
Sigma-Aldrich

Sigma-Aldrich

L9530

Linoleic Acid-Albumin from bovine serum albumin

Bovine Serum Albumin lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A6003

Bovine Serum Albumin

Sigma-Aldrich

Sigma-Aldrich

O3008

Oleic Acid-Albumin from bovine serum

Sigma-Aldrich

Sigma-Aldrich

L8384

Linoleic Acid-Albumin from bovine serum albumin

form

liquid

form

lyophilized powder

form

liquid

form

lyophilized powder

concentration

100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)

concentration

-

concentration

100 mg/mL BSA in DPBS, 90-120 mg/mL protein (biuret)

concentration

-

technique(s)

cell culture | mammalian: suitable

technique(s)

microbiological culture: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

impurities

≤400 EU/mL endotoxin

impurities

≤0.01% fatty acid (GC)

impurities

endotoxin, tested

impurities

≤50 EU/mg endotoxin

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

General description

Fatty Acid-Albumin - complexes have been employed as alternative sources of lipids in the development of serum-free media. Fatty acids bind to serum proteins in high proportions. Such proteins may release beneficial fatty acids and bind those that are inhibitory. Linoleic acid, a precursor of prostaglandins bound to BSA is beneficial to the growth of a variety of cell types (e.g., BHK, hybridoma). It also improves its stability and solubility in culture media.

Application

Linoleic Acid-Albumin from bovine serum albumin is used in the cell line culture and cell differentiation medium.
Linoleic Acid-Albumin from bovine serum albumin is used in the cell line culture and cell differentiation medium.

Other Notes

Recommended usage: 1 ml/100 ml medium

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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Chiu-Fang Liang et al.
IUBMB life, 54(5), 275-279 (2003-02-18)
Epidermal growth factor (EGF) was reported to regulate triacyl glycerol synthesis in various cells. Linoleic acid and its metabolites were thought to modulate the signal transduction of growth factors. This study determined whether linoleic acid regulated the effect of EGF
Doreen Reichert et al.
Cells, 10(4) (2021-05-01)
Chronic liver diseases are associated with excessive deposition of extracellular matrix proteins. This so-called fibrosis can progress to cirrhosis and impair vital functions of the liver. We examined whether the receptor tyrosine kinase (RTK) class III inhibitor Crenolanib affects the
Dong-Hwan Kim et al.
Lipids, 55(2), 163-171 (2020-02-15)
The regulation of adipocyte differentiation is an important factor for production efficiency and meat quality in the poultry industry. The purpose of this study was to develop a new in vitro model of adipogenic differentiation of chicken embryonic fibroblasts (CEF).
Cécile Coste et al.
PloS one, 12(7), e0177962-e0177962 (2017-07-07)
Adult neural crest stem-derived cells (NCSC) are of extraordinary high plasticity and promising candidates for use in regenerative medicine. Several locations such as skin, adipose tissue, dental pulp or bone marrow have been described in rodent, as sources of NCSC.
Li-Ping Liu et al.
STAR protocols, 1(1), 100004-100004 (2020-10-29)
Melanocytes, derived from neural crest cells, are involved in melanin production. This protocol describes a method to generate induced melanocytes (iMelanocytes) from human induced pluripotent stem cells (iPSCs) using a suspension culture system, which considerably improves the differentiation efficiency. The
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Articles

Linoleic acid in Cell Culture

Importance and uses of linoleic acid in serum-free eukaryotic, including hybridoma and Chinese Hamster Ovary (CHO) cell, cultures

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