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A6003

Sigma-Aldrich

Bovine Serum Albumin

lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)

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Synonym(s):
Albumin bovine serum, BSA, Bovine albumin
CAS Number:
EC Number:
MDL number:

biological source

bovine

Quality Level

Assay

≥96% (agarose gel electrophoresis)
≥96%

form

lyophilized powder

mol wt

~66 kDa

purified by

cold ethanol fractionation

packaging

poly bottle of

origin

USA origin

free fatty acid content

≤0.01%

technique(s)

microbiological culture: suitable

impurities

≤0.01% fatty acid (GC)

pH

7

solubility

water: soluble (50 mg/ml)

UniProt accession no.

application(s)

hematology

foreign activity

BT virus, none detected
VSV virus, none detected

storage temp.

2-8°C

Gene Information

bovine ... ALB(280717)

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This Item
A8806A1470A2058
Bovine Serum Albumin lyophilized powder, essentially fatty acid free, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A6003

Bovine Serum Albumin

Bovine Serum Albumin fatty acid free, low endotoxin, lyophilized powder, BioReagent, suitable for cell culture, ≥96% (agarose gel electrophoresis)

Sigma-Aldrich

A8806

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, low endotoxin, BioReagent, suitable for cell culture, ≥98% (agarose gel electrophoresis)

Sigma-Aldrich

A1470

Bovine Serum Albumin

Bovine Serum Albumin lyophilized powder, essentially IgG-free, low endotoxin, BioReagent, suitable for cell culture

Sigma-Aldrich

A2058

Bovine Serum Albumin

assay

≥96% (agarose gel electrophoresis)

assay

≥96% (agarose gel electrophoresis)

assay

≥98% (agarose gel electrophoresis)

assay

≥95% (agarose gel electrophoresis)

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

form

lyophilized powder

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

mol wt

~66 kDa

purified by

cold ethanol fractionation

purified by

cold ethanol fractionation

purified by

heat shock fractionation

purified by

chromatography

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

packaging

poly bottle of

General description

BSA is a known carrier of fatty acids (FA). Thus control over the specific FA′s for culture of cell lines is important, as different cell lines can be differentially sensitive to particular fatty acids. Fatty acid-free BSA thus is a useful tool for cell culture experiments where the specific fatty acid content must be tightly controlled, to allow researchers to use particular fatty acids specific to their cell lines. Fatty acid-free albumin also allows for optimal and maximum binding sites for adding specific fatty acids to use in cell culture. The use of FA-free BSA also mitigates concerns about endogenous FA′s potentially in non-FA-free BSA.
Bovine serum albumin (BSA) is a highly abundant plasma protein that assists in the regulation of the colloidal osmotic pressure of blood. BSA is used in many different applications, such as cell culture, immunochemistry, and protein quantitation.

This BSA product is specifically tested for very low FA content / fatty acid free status.

Biochem/physiol Actions

Certain conformational and primary-sequence epitopes of BSA are suspected allergens in human beef and milk allergies.

Features and Benefits

  • Cold ethanol fractionated
  • Essentially fatty acid-free

Preparation Note

Often referred to as Cohn fraction V; this product is prepared by a modified method of the Cohn cold ethanol fractionation method.
Serum albumin may be referred to as Fraction V. This naming convention is taken from the original Cohn method of fractionating serum proteins using cold ethanol precipitation. Serum albumin was found in the fifth ethanol fraction using Cohn′s method. Since then, the term "Fraction V" has been used by some to describe serum albumin regardless of the method of preparation. Others have used this term to describe serum albumin purified by ethanol fractionation methods that have been highly modified since the original Cohn method was described. Sigma-Aldrich manufactures and distributes serum albumins purified from a variety of primary methods including the true Cohn fractionation method, modified ethanol fractionation methods, heat shock and chromatography. Additional purification steps may include crystallization or charcoal filtration.

Storage Class Code

11 - Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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25G
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705578-5MG-PW

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MMYOMAG-74K-13

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Related Content

Albumin from Bovine Serum Frequently Asked Questions (FAQs)

Learn about bovine serum albumin (BSA) structure, molecular weight, function, solubility, how BSA is made, purification methods, applications and purchasing.

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