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I3146

Sigma-Aldrich

ITS Liquid Media Supplement (100×)

liquid, sterile-filtered, BioReagent, suitable for cell culture

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Synonym(s):
Human ITS Liquid Media, Insulin-transferrin-sodium selenite
NACRES:
NA.75

sterility

sterile-filtered

Quality Level

500

product line

BioReagent

form

liquid

technique(s)

cell culture | mammalian: suitable
single cell analysis: suitable

impurities

endotoxin, tested

shipped in

ambient

storage temp.

2-8°C

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ITS Liquid Media Supplement (100×)

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form

liquid

form

liquid

form

liquid

form

liquid

technique(s)

cell culture | mammalian: suitable, single cell analysis: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable, cell culture | stem cell: suitable

technique(s)

cell culture | mammalian: suitable

impurities

endotoxin, tested

impurities

endotoxin, tested

impurities

-

impurities

endotoxin, tested

shipped in

ambient

shipped in

ambient

shipped in

ambient

shipped in

ambient

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

2-8°C

Application

ITS Liquid Media Supplement (100×) has been used as a media supplement to culture:
  • α mouse liver 12 (AML-12) cells
  • rat fetal testis explants
  • human dental pulp stem cells (DPSC)

Biochem/physiol Actions

  • Insulin: a polypeptide hormone that promotes the uptake of glucose and amino acids and may owe an observed mitogenic effect to this property.
  • Transferrin: an iron-transport protein.
  • Selenium: an essential trace element normally provided by serum.

Components

Contains 1.0 mg/ml recombinant human insulin, 0.55 mg/ml human transferrin (substantially iron-free), and 0.5 μg/ml sodium selenite at the 100x concentration.

Other Notes

ITS is a mixture of recombinant human insulin, human transferrin, and sodium selenite. It is a general cell supplement designed for use in non-complex media (e.g. MEM, RPMI-1640) and complex media (e.g. Ham?s F-12, DME/F-12, MEM) with sodium pyruvate.

Preparation Note

Prepared in Earle′s Balanced Salt Solution (EBSS) without phenol red.

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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