Skip to Content
MilliporeSigma
All Photos(1)

Key Documents

E0760

Sigma-Aldrich

Endothelial cell growth supplement from bovine pituitary

Bioreagent, powder, suitable for cell culture

Synonym(s):

ECGS, Endothelial Mitogen

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
12352202
NACRES:
NA.77

product name

Endothelial cell growth supplement from bovine pituitary, ECGS, suitable for cell culture, BioReagent

biological source

bovine pituitary glands

Quality Level

product line

BioReagent

form

powder

potency

2-300 μg/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

brown to tan

solubility

water: soluble to hazy, red to red-brown (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

General description

Endothelial cell growth supplement (ECGS) is an extract of bovine pituitary glands containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of a published method. It is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Application

Endothelial cell growth supplement from bovine pituitary has been used:
  • as a component in Roswell park memorial institute (RPMI) 1640 medium for endothelial cell culture
  • as a supplement in medium 199 for endothelial cell culture
  • as a supplement in Dulbecco′s modified eagle medium (DMEM):Ham′s F-12
  • as a supplement in Iscove′s modified Dulbecco′s medium (IMDM) for human umbilical vein endothelial cells (HUVEC) culture

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

pictograms

Health hazard

signalword

Warning

hcodes

Hazard Classifications

Repr. 2

Storage Class

10 - Combustible liquids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

TGF-beta 1 released from activated platelets can induce TNF-stimulated human brain endothelium apoptosis: a new mechanism for microvascular lesion during cerebral malaria
Wassmer SC, et al.
Journal of Immunology, 176(2), 1180-1184 (2006)
Guihua Wu et al.
Pharmaceutical biology, 59(1), 1388-1401 (2021-10-20)
Genistein (Gen) has shown protective effects against ageing process. To explore the role of Gen on the senescence of H2O2-induced human umbilical vein endothelial cells (HUVECs) and investigate the possible mechanism. HUVECs were treated with different concentrations of H2O2 (50
Surface staining and cytotoxic activity of heat-shock protein 60 antibody in stressed aortic endothelial cells.
Xu Q, et al.
Circulation Research, 75(6), 1078-1085 (1994)
Autoantibodies against heat shock protein 60 mediate endothelial cytotoxicity.
Schett G, et al.
The Journal of Clinical Investigation, 96(6), 2569-2577 (1995)
Pei-Yu Chou et al.
Food science & nutrition, 8(2), 1093-1103 (2020-03-10)
This study investigated the effects of methanol extract Magnolia officinalis (MEMO) on baroreceptor reflex sensitivity (BRS) in the hypercholesterolemic rabbits and the involved molecular mechanisms. Male New Zealand white rabbits were randomly divided into Control (normal diet), Cholesterol (0.5% w/w

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service