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E2759

Sigma-Aldrich

Endothelial cell growth supplement from bovine neural tissue

ECGS, suitable for cell culture

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Synonym(s):
ECGS, Endothelial Mitogen
NACRES:
NA.77

biological source

bovine neuroblastoma

Quality Level

form

powder

potency

2-200 μg/mL ED50/EC50

quality

endotoxin tested

packaging

pkg of 15 and 5X15 mg

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

impurities

≤20 EU/vial (LAL test)

color

tan to brown

solubility

water: soluble (1 vial reconstituted with 1 ml 0.85% NaCl)

storage temp.

2-8°C

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This Item
E0760E4127F3133
form

powder

form

powder

form

lyophilized powder

form

lyophilized powder

potency

2-200 μg/mL ED50/EC50

potency

2-300 μg/mL ED50/EC50

potency

0.1-10 ng/mL ED50/EC50

potency

1-100 ng/mL

packaging

pkg of 15 and 5X15 mg

packaging

pkg of 15 mg

packaging

pkg of 0.1 and 5X0.1 mg

packaging

pkg of 10 and 5X10 μg

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

storage condition

avoid repeated freeze/thaw cycles

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

technique(s)

cell culture | mammalian: suitable

General description

Endothelial cell growth supplement (ECGS) comprises acidic fibroblast growth factor (aFGF) and basic FGF (bFGF).

Application

Endothelial cell growth supplement from bovine neural tissue has been used as a supplement in Dulbecco′s modified Eagle′s medium supplement and F-12K media supplement for human umbilical vein endothelial cells culture.

Biochem/physiol Actions

Endothelial cell growth supplement (ECGS) improves proliferation of human saphenous vein endothelial cells (HSVEC) and human umbilical vein endothelial cells (HUVEC). It coordinates with interleukin 1 (IL-1) and mediates matrix remodelling in vascular smooth muscle cells (VSMCs). It is essential for endothelial cells proliferation and adipose-derived stem cell (ASC) differentiation. An extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. ECGS has also been reported to be beneficial as a media supplement for the fusion and growth of hybridoma cells in monoclonal antibody production.

Preparation Note

ECGS is an extract of bovine neural tissue containing growth promoting factors for vascular endothelial cells of mammalian origin. Endothelial cell growth supplement is prepared using a modification of the method of Maciag, et al., and is lyophilized from a sterile solution containing NaCl and streptomycin sulfate.

Analysis Note

The proliferative activity of endothelial cell growth supplement (ECGS) is tested in culture using fetal bovine heart endothelial cells (ATCC CRL 1395) seeded at low density. The EC50 is defined as the effective concentration of growth factor that elicits a 50% increase in cell growth in a cell based bioassay.

Storage Class Code

13 - Non Combustible Solids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

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T Maciag et al.
The Journal of biological chemistry, 257(10), 5333-5336 (1982-05-25)
Endothelial cell growth factor (ECGF) is a neural-derived mitogen capable of stimulating the proliferation of quiescent populations of human umbilical vein endothelial cells (HUV-EC). ECGF has been isolated from bovine hypothalamus in two forms: a high molecular weight from (high
T Maciag et al.
Proceedings of the National Academy of Sciences of the United States of America, 76(11), 5674-5678 (1979-11-01)
Extracts of bovine hypothalamus were found to contain a significant level of mitogenic activity when tested in a Swiss 3T3 cell [3H]dThd incorporation assay and in a human umbilical vein endothelial cell growth assay. The mitogenic activity responsible for 3T3
Coculture of mesenchymal stem cells and endothelial cells enhances host tissue integration and epidermis maturation through AKT activation in gelatin methacryloyl hydrogel-based skin model
Zhang X, et al.
Acta Biomaterialia, 59(4), 317-326 (2017)
Soluble adhesion molecules correlate with surface expression in an in vitro model of endothelial activation
Kjaergaard AG, et al.
Basic and Clinical Pharmacology and Toxicology, 113(4), 273-279 (2013)
KML001 displays vascular disrupting properties and irinotecan combined antitumor activities in a murine tumor model
Moon CH, et al.
Testing, 8(1), e53900-e53900 (2013)

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