A9434

Ammonium chloride

for molecular biology, suitable for cell culture, ≥99.5%

• Synonym(s): Salmiac
• Linear Formula: NH₄Cl
• CAS Number: 12125-02-9
• Molecular Weight: 53.49
• Beilstein: 4371014
• EC Number: 235-186-4
• MDL number: MFCD00011420
• UNSPSC Code: 12352302
• eCl@ss: 38050207
• PubChem Substance ID: 24891452
• NACRES: NA.31

Properties

• grade: for molecular biology
• vapor density: 1.9 (vs air)
• vapor pressure: 1 mmHg ( 160.4 °C)
• Assay: ≥99.5%
• form: powder or crystals
• technique(s): cell culture | mammalian: suitable
• mp: 340 °C (subl.) (lit.)
• anion traces: phosphate (PO₄^3-): ≤2 ppm; sulfate (SO₄^2-): ≤20 ppm
• cation traces: Fe: ≤2 ppm; heavy metals (as Pb): ≤5 ppm
• foreign activity: DNase, RNase, protease, none detected
• SMILES string: N.Cl
• InChI: 1S/ClH.H3N/h1H;1H3
• InChI key: NLXLAEXVIDQMFP-UHFFFAOYSA-N

Description

Application

Ammonium chloride has been used:

• as an inhibitor of endosome acidification in vero cells
• as an autophagy inhibitor in vascular smooth muscle cell (VSMC)
• in the depletion and lysis of erythrocytes in testis parenchyma homogenate

Biochem/physiol Actions

Ammonium chloride is one of the three principal components of the nitrogen cycle. Ammonium chloride administration promotes creatinine and urea clearance andinduces metabolic acidosis in mice. Excess of ammonium chloride inhibits Kreb′s pathway in brain and depletes ATP in astrocytes. Ammonium chloride inhibits acidification in the endosome-lysosome system. Ammonium chloride is a hemolysing agent used for the lysis of erythrocytes.

Safety Information

• Pictograms: GHS07
• Signal Word: Warning
• Hazard Statements: H302,H319
• Precautionary Statements: P264 - P280 - P301 + P312 - P305 + P351 + P338 - P337 + P313 - P501
• Hazard Classifications: Acute Tox. 4 Oral - Eye Irrit. 2
• Storage Class Code: 13 - Non Combustible Solids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves