추천 제품
생물학적 소스
rabbit
Quality Level
항체 형태
purified immunoglobulin
항체 생산 유형
primary antibodies
클론
polyclonal
종 반응성
mouse, rat, hamster, human
제조업체/상표
Upstate®
기술
electrophoretic mobility shift assay: suitable
immunohistochemistry (formalin-fixed, paraffin-embedded sections): suitable
immunoprecipitation (IP): suitable
western blot: suitable
동형
IgG
NCBI 수납 번호
UniProt 수납 번호
배송 상태
wet ice
타겟 번역 후 변형
phosphorylation (pSer133)
유전자 정보
human ... CREB1(1385)
일반 설명
CREB is a β-ZIP transcription factor that activates target genes through cAMP response elements. CREB is able to mediate signals from numerous physiological stimuli, resulting in regulation of a broad array of cellular responses. While CREB is expressed in numerous tissues, it plays a large regulatory role in the nervous system. CREB is believed to play a key role in promoting neuronal survival, precursor proliferation, neurite outgrowth and neuronal differentiation in certain neuronal populations. CREB promotes outgrowth and differentiation as a mediator of neurotrophin pathways. CREB is able to selectively activate numerous downstream genes through interactions with different dimerization partners. CREB is activated by phosphorylation at Ser133 by various signaling pathways including Erk, Ca2+ and stress signaling.
특이성
Recognizes p43 phosphorylated CREB. May also recognize p30 and p38 kDa proteins. The p30 & p38 proteins may be phosphorylated CREM and ATF-1 respectively, since the two proteins share significant homology to the phosphorylated CREB immunizing peptide.
면역원
Epitope: phospho-Ser133
KLH-conjugated synthetic peptide corresponding to the amino acids including and encompassing phosphorylated Ser133 of CREB.
애플리케이션
Immunoprecipitation:
10 μL of a previous lot immunoprecipitated phosphorylated CREB from 500 μg of cells treated with 50 μM forskolin.
Immunohistochemistry:
A 1:1000 dilution of previous lots detected phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.
Gel Shift (EMSA):
This antibody has been shown to gel shift by independent labs.
10 μL of a previous lot immunoprecipitated phosphorylated CREB from 500 μg of cells treated with 50 μM forskolin.
Immunohistochemistry:
A 1:1000 dilution of previous lots detected phosphorylated CREB in formalin-fixed paraffin-embedded normal and ischemic rat brain sections.
Gel Shift (EMSA):
This antibody has been shown to gel shift by independent labs.
Research Category
Signaling
Signaling
Research Sub Category
Nuclear Receptors
Nuclear Receptors
Use Anti-phospho-CREB (Ser133) Antibody (rabbit polyclonal antibody) is highly published and validated in EMSA, IHC,IHC(P), IP, WB to detect phospho-CREB (Ser133) also known as active transcription factor CREB, cAMP responsive element binding protein 1.
품질
Evaluated by western blot on lysates from mouse NIH/3T3 cells treated with 50 mM forskolin.
Western Blot Analysis: This antibody detected CREB when phosphorylated on Ser133 at 1:1,000 dilution in forskolin treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
Western Blot Analysis: This antibody detected CREB when phosphorylated on Ser133 at 1:1,000 dilution in forskolin treated NIH/3T3 lysates via SDS-PAGE and transferred to PVDF (Immobilon-P).
표적 설명
Detects CREB only when phosphorylated on Ser133 (~43 kDa). May recognize ATF-1 (~38 kDa) and CREM (~30 kDa) as the two proteins share significant homology to the phosphorylated immunizing peptide.
물리적 형태
Format: Purified
Purified by protein A-Sepharose followed by immunoadsorbant chromatography using an unphosphorylated CREB affinity gel column.
Purified rabbit IgG in buffer containing 0.1 M Tris-Glycine (pH 7.4) 15 mM NaCl, and 0.05% sodium azide with 30% glycerol.
저장 및 안정성
Stable for 1 year at -20°C from date of receipt.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
Handling Recommendations:
Upon receipt, and prior to removing the cap, centrifuge the vial and gently mix the solution. Aliquot into microcentrifuge tubes and store at -20°C.
Avoid repeated freeze/thaw cycles, which may damage IgG and affect product performance.
분석 메모
Control
Forskolin-stimulated WI-38 nuclear extracts.
Forskolin-stimulated WI-38 nuclear extracts.
기타 정보
Concentration: Please refer to the Certificate of Analysis for the lot-specific concentration.
법적 정보
UPSTATE is a registered trademark of Merck KGaA, Darmstadt, Germany
면책조항
Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.
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Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 1
Flash Point (°F)
Not applicable
Flash Point (°C)
Not applicable
시험 성적서(COA)
제품의 로트/배치 번호를 입력하여 시험 성적서(COA)을 검색하십시오. 로트 및 배치 번호는 제품 라벨에 있는 ‘로트’ 또는 ‘배치’라는 용어 뒤에서 찾을 수 있습니다.
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