07-1404

Anti-GLUT-4 Antibody, C-terminus

from rabbit, purified by affinity chromatography

• 동의어(들): Solute carrier family 2, facilitated glucose transporter member 4, Glucose transporter type 4, insulin-responsive, GLUT-4
• UNSPSC 코드: 12352203
• eCl@ss: 32160702
• NACRES: NA.41

속성

• 생물학적 소스: rabbit
• Quality Level: 100
• 항체 형태: affinity isolated antibody
• 항체 생산 유형: primary antibodies
• 클론: polyclonal
• 정제법: affinity chromatography
• 종 반응성: mouse, human
• 종 반응성(상동성에 의해 예측): rat (based on 100% sequence homology)
• 기술: immunohistochemistry: suitable; western blot: suitable
• NCBI 수납 번호: NP_001033
• UniProt 수납 번호: P14672
• 배송 상태: wet ice
• 타겟 번역 후 변형: unmodified
• 유전자 정보: human ... SLC2A4(6517)

설명

일반 설명

The protein called GLUT-4, or more correctly Solute carrier family 2, facilitated glucose transporter member 4 and encoded by the human gene SLC2A4/GLUT4 is a primary insulin regulated facilitative glucose transporter found primarily in skeletal and cardiac muscle, as well as brown and white fat but is also expressed in other tissues such as brain. In the brain GLUT4 is found exclusively in neurons. GLUT-4 is facilitative because it is not always present on the cell plasma membrane. GLUT-4 only localizes to the plasma membrane upon insulin induction. GLUT-4 is localized to the perinuclear region in resting cells. Mutations in GLUT-4 are a cause of one form of non-insulin dependent diabetes mellitus. GLUT4 function as well as its transcriptional regulation is highly conserved throughout the vertebrate species.

특이성

Glucose transporter 4 (GLUT-4) is a protein present in rat skeletal muscle, heart and adipose tissues. GLUT-4 is highly conserved between species, and the peptide sequence selected for production of this antibody is identical in rat, human (amino acids 498-510), and mouse. This antibody shows no cross-reactivity of HepG2 type transporter from rat brain or other glucose transporters.

면역원

Epitope: C-terminus

KLH-conjugated linear peptide corresponding to the C-terminus of human GLUT-4.

애플리케이션

Detect GLUT-4 using this Anti-GLUT-4 Antibody, C-terminus validated for use in WB, IH(P), ELISA.

Immunohistochemistry Analysis: A 1:50-2,000 dilution from a representative lot detected GLUT-4 in human cardiac myocytes and human skeletal muscle myocytes.

Research Category
Signaling

Research Sub Category
Insulin/Energy Signaling

품질

Evaluated by Western Blotting in NIH3T3 cell lysate.

Western Blotting Analysis: A 1:1,000 dilution of this antibody detected GLUT-4 in 10 µg of NIH3T3 cell lysate.

표적 설명

~58 kDa observed

결합

Replaces: AB1346

물리적 형태

Affinity purified

Purified rabbit polyclonal in buffer containing PBS with 0.05% sodium azide.

저장 및 안정성

Stable for 1 year at 2-8°C from date of receipt.

분석 메모

Control
NIH3T3 cell lysate

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 2