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Merck
모든 사진(1)

주요 문서

AB5612

Sigma-Aldrich

Anti-Citrulline Antibody

serum, Chemicon®

동의어(들):

CCP

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About This Item

UNSPSC 코드:
12352203
eCl@ss:
32160702
NACRES:
NA.41
클론:
polyclonal
application:
ELISA
IHC
기술:
ELISA: suitable
immunohistochemistry: suitable
citations:
2

생물학적 소스

rabbit

Quality Level

항체 형태

serum

항체 생산 유형

primary antibodies

클론

polyclonal

종 반응성(상동성에 의해 예측)

mammals

제조업체/상표

Chemicon®

기술

ELISA: suitable
immunohistochemistry: suitable

적합성

not suitable for immunohistochemistry (Paraffin)

배송 상태

wet ice

타겟 번역 후 변형

unmodified

특이성

Citrulline in glutaraldehyde fixed tissues or cells.



The cross-reactivities were determined using an ELISA test by competition experiments with the following compounds:



Compound Cross-reactivity ratio

Citrulline-G-BSA 1

Arginine-G-BSA 1/>100,000

Glutamine-G-BSA 1/>100,000

Ornithine-G-BSA 1/>100,000



Abbreviations:



(G) Glutaraldehyde

(BSA) Bovine Serum Albumin

면역원

Citrulline-Glutaraldehyde-BSA

애플리케이션

Anti-Citrulline Antibody is a Rabbit Polyclonal Antibody for the detection of Citrulline & has been validated in ELISA & IHC.
Immunohistochemistry: 1:1,000-1:2,500 glutaraldehyde required for reactivity. (0.5%-2.5% is typical; fluorescence detection is not recommended. Blocking is with 3% normal serum with 0.1-0.2% triton X-100 usually. Primary antibody is diluted only in TBS or PBS with out blocking or triton X-100. Incubations are usually 3 hours 37C or 4C overnight. Detection is by ABC or PAP for best results. Not recommended for paraffin embedded tissues.

ELISA: 1:2500-1:5000

Optimal working dilutions must be determined by the end user.
Research Category
Epigenetics & Nuclear Function
Research Sub Category
Chromatin Biology

물리적 형태

Rabbit antiserum. Liquid with 0.05% sodium azide

저장 및 안정성

Maintain at -20°C in undiluted aliquots for up to 6 months after date of receipt. Avoid repeated freeze/thaw cycles.

법적 정보

CHEMICON is a registered trademark of Merck KGaA, Darmstadt, Germany

면책조항

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

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Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


시험 성적서(COA)

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문서 라이브러리 방문

Charlotte Thålin et al.
Journal of thrombosis and haemostasis : JTH, 18(10), 2732-2743 (2020-07-13)
Recent data propose a diagnostic and prognostic capacity for citrullinated histone H3 (H3Cit), a marker of neutrophil extracellular traps (NETs), in pathologic conditions such as cancer and thrombosis. However, current research is hampered by lack of standardized assays. We aimed
Priyanka Sharma et al.
Molecular cell, 73(1), 84-96 (2018-11-26)
The post-translational modification of key residues at the C-terminal domain of RNA polymerase II (RNAP2-CTD) coordinates transcription, splicing, and RNA processing by modulating its capacity to act as a landing platform for a variety of protein complexes. Here, we identify

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