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Merck
모든 사진(4)

문서

S2002

Sigma-Aldrich

Sodium azide

ReagentPlus®, ≥99.5%

동의어(들):

Hydrazoic acid sodium salt

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About This Item

Linear Formula:
NaN3
CAS Number:
Molecular Weight:
65.01
EC Number:
MDL number:
UNSPSC 코드:
12352201
PubChem Substance ID:
NACRES:
NA.21

생물학적 소스

synthetic (inorganic)

Quality Level

제품 라인

ReagentPlus®

분석

≥99.5%

형태

powder

저장 조건

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

기술

cell based assay: suitable
immunohistochemistry: suitable

색상

white

mp

370-425 °C

solubility

soluble 65 g/L at 20 °C (completely)

적합성

suitable for chromatography
suitable for derivatization

응용 분야

sample preparation

SMILES string

[Na]N=[N+]=[N-]

InChI

1S/N3.Na/c1-3-2;/q-1;+1

InChI key

PXIPVTKHYLBLMZ-UHFFFAOYSA-N

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일반 설명

Sodium Azide (NaN3) is a potent inhibitor – a water-soluble crystalline powder that′s odorless. Widely recognized for its versatile applications, it plays a pivotal role across molecular biology, cell culture, and biochemical research. Sodium azide is commonly used as a bacteriostatic preservative in aqueous laboratory reagents and biological fluids. It is also a metabolic inhibitor, which inhibits oxidative phosphorylation. In cell culture, it maintains the integrity of your cell lines by preventing microbial contamination. Its application extends to molecular biology, where it aids in preserving nucleic acids for downstream analysis, ensuring the fidelity of your results.

애플리케이션

Baylis-Hillman acetates such as ethyl 2-(acetoxyphenylmethyl)acrylate can undergo nucleophilic displacement by sodium azide in aqueous medium to form ethyl (E)-2-azidomethyl-3-phenylpropenoate.
Sodium azide is suitable for use in histopathology to prepare and store tissue samples. Also used as a component of staining buffer (HEPES buffer) for whole mount immunolabelling.
Catalyst for:

  • Oxidative decarboxylation
  • Michael addition reactions

Reagent for synthesis of
  • Blue fluorescent copolymers
  • Metal phosphonates
  • Arenes via aminations

생화학적/생리학적 작용

Sodium azide is clinically used as a preservative in diluting fluid for counting red blood cells. It prevents capping and internalization of fluorescent surface-bound antibodies in various scientific applications, including flow cytometry.

기타 정보

For additional information on our range of Biochemicals, please complete this form.

법적 정보

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

스플래시 보호용 추천 장갑

호환 제품

제품 번호
설명
가격

픽토그램

Skull and crossbonesHealth hazardEnvironment

신호어

Danger

유해 및 위험 성명서

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2 Oral

표적 기관

Brain

보충제 위험성

Storage Class Code

6.1A - Combustible acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point (°F)

Not applicable

Flash Point (°C)

Not applicable


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A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination
Patricia A Arndt , George Garratty
Transfusion Medicine Reviews, 24(3), 174-192 (2010)
Nucleophilic displacement by azide and cyanide on Baylis-Hillman acetates in water.
Yadav JS, et al.
Tetrahedron Letters, 46(16), 2761-2763 (2005)
Sodium azide, a bacteriostatic preservative contained in commercially available laboratory reagents, influences the responses of human platelets via the cGMP/PKG/VASP pathway.
Russo I, et al.
Clinical Biochemistry, 41(4), 343-349 (2008)
Calcium uptake by rat kidney mitochondria.
DeLuca HF and Engstrom GW.
Proceedings of the National Academy of Sciences of the USA, 47(11), 1744-1750 (1961)
Three-dimensional cellular visualization in mouse apical periodontitis using combined whole-mount staining and optical tissue clearing
Kento Tazawa , Hajime Sasaki
Journal of oral biosciences, 65, 132-135 (2023)

프로토콜

Antibody purification protocols yield preparations containing endogenous IgG alongside specific antibodies.

간접 ELISA 및 포획 ELISA 실시에 관한 상세한 지침을 제공하는 ELISA 프로토콜 샌드위치 ELISA 및 간접 ELISA 실험을 실시하기 위한 권장 제품과 기법에 대해 자세히 알아봅니다.

Detailed ELISA protocols cover indirect and capture ELISA techniques, recommending products for sandwich and indirect ELISA experiments.

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