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S2002

Sigma-Aldrich

Sodium azide

ReagentPlus®, ≥99.5%

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Synonym(s):
Hydrazoic acid sodium salt
Linear Formula:
NaN3
CAS Number:
Molecular Weight:
65.01
EC Number:
MDL number:
PubChem Substance ID:
NACRES:
NA.21

biological source

synthetic (inorganic)

Quality Level

product line

ReagentPlus®

Assay

≥99.5%

form

powder

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

color

white

mp

370-425 °C

solubility

soluble 65 g/L at 20 °C (completely)

SMILES string

[Na]N=[N+]=[N-]

InChI

1S/N3.Na/c1-3-2;/q-1;+1

InChI key

PXIPVTKHYLBLMZ-UHFFFAOYSA-N

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1 of 4

This Item
S803271290769320
Sodium azide ReagentPlus®, ≥99.5%

Sigma-Aldrich

S2002

Sodium azide

-
Sodium azide BioXtra

Sigma-Aldrich

S8032

Sodium azide

Premium Grade
Sodium azide purum p.a., ≥99.0% (T)

Sigma-Aldrich

71290

Sodium azide

-
Sodium azide ≥99%, ultra dry

Sigma-Aldrich

769320

Sodium azide

-
assay

≥99.5%

assay

99.8%

assay

≥99.0% (T)

assay

≥99%, 99.0-101.0% (RT)

form

powder

form

powder or crystals

form

crystals

form

solid

storage condition

(Tightly closed. Dry. Keep in a well-ventilated place. Do not store near acids.)

storage condition

-

storage condition

-

storage condition

-

color

white

color

-

color

-

color

-

mp

370-425 °C

mp

370-425 °C

mp

-

mp

-

General description

Sodium Azide (NaN3) is a potent inhibitor – a water-soluble crystalline powder that′s odorless. Widely recognized for its versatile applications, it plays a pivotal role across molecular biology, cell culture, and biochemical research. Sodium azide is commonly used as a bacteriostatic preservative in aqueous laboratory reagents and biological fluids. It is also a metabolic inhibitor, which inhibits oxidative phosphorylation. In cell culture, it maintains the integrity of your cell lines by preventing microbial contamination. Its application extends to molecular biology, where it aids in preserving nucleic acids for downstream analysis, ensuring the fidelity of your results.

Application

Baylis-Hillman acetates such as ethyl 2-(acetoxyphenylmethyl)acrylate can undergo nucleophilic displacement by sodium azide in aqueous medium to form ethyl (E)-2-azidomethyl-3-phenylpropenoate.
Sodium azide is suitable for use in histopathology to prepare and store tissue samples. Also used as a component of staining buffer (HEPES buffer) for whole mount immunolabelling.
Catalyst for:

  • Oxidative decarboxylation
  • Michael addition reactions

Reagent for synthesis of
  • Blue fluorescent copolymers
  • Metal phosphonates
  • Arenes via aminations

Biochem/physiol Actions

Sodium azide is clinically used as a preservative in diluting fluid for counting red blood cells. It prevents capping and internalization of fluorescent surface-bound antibodies in various scientific applications, including flow cytometry.

Legal Information

ReagentPlus is a registered trademark of Merck KGaA, Darmstadt, Germany

comparable product

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Description
Pricing

suggested gloves for splash protection

Signal Word

Danger

Hazard Classifications

Acute Tox. 1 Dermal - Acute Tox. 2 Inhalation - Acute Tox. 2 Oral - Aquatic Acute 1 - Aquatic Chronic 1 - STOT RE 2 Oral

Target Organs

Brain

Supplementary Hazards

Storage Class Code

6.1A - Combustible, acute toxic Cat. 1 and 2 / very toxic hazardous materials

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Sodium azide, a bacteriostatic preservative contained in commercially available laboratory reagents, influences the responses of human platelets via the cGMP/PKG/VASP pathway.
Russo I, et al.
Clinical Biochemistry, 41(4), 343-349 (2008)
Nucleophilic displacement by azide and cyanide on Baylis-Hillman acetates in water.
Yadav JS, et al.
Tetrahedron Letters, 46(16), 2761-2763 (2005)
A critical review of published methods for analysis of red cell antigen-antibody reactions by flow cytometry, and approaches for resolving problems with red cell agglutination
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Protocols

Antibody Purification using Protein A, Protein G, or Protein L Agarose protocol is designed as a quick purification method for antibodies from mammalian sera, ascites, and cell culture supernatants. It should be noted that if the starting material is serum or ascites the final preparation will contain endogenous host IgG as well as specific antibodies. In general, the presence of this endogenous IgG should not interfere with assays using the antibodies.

ELISA protocols providing detailed instructions on performing indirect ELISA and Capture ELISA . Learn more about recommended products and techniques for performing both sandwich ELISA and indirect ELISA experiments.

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