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Merck
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B5655

Sigma-Aldrich

BCIP®/NBT

SIGMAFAST™, alkaline phosphatase substrate, chromogenic, tablet

동의어(들):

BCIP/NBT Substrate, BCIP®/NBT Alkaline Phosphatase Substrate

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About This Item

UNSPSC 코드:
12352204
NACRES:
NA.83
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제품명

SIGMAFAST™ BCIP®/NBT, tablet

양식

tablet

Quality Level

200

solubility

water: 10 mL, clear, yellow

저장 온도

−20°C

관련 카테고리

애플리케이션

SIGMAFAST BCIP®/NBT has been used as a substrate
  • for the anti-mouse IgG conjugated alkaline phosphatase in immunoblotting[1]
  • for alkaline phosphatase in the histochemical staining of primary osteoblasts[2]
  • for alkaline phosphatase staining in bone marrow stromal cells[3]

재구성

Each tablet dissolved in 10 ml deionized water yields a ready-to-use buffered solution containing BCIP/NBT, pH 9.5.

법적 정보

BCIP is a registered trademark of Merck KGaA, Darmstadt, Germany
SIGMAFAST is a trademark of Sigma-Aldrich Co. LLC

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

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시험 성적서(COA)

Lot/Batch Number
0000411613
0000411612
0000411612
0000411613
0000411215

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문서 라이브러리에서 최근에 구매한 제품에 대한 문서를 찾아보세요.

문서 라이브러리 방문

Sialic acid on herpes simplex virus type 1 envelope glycoproteins is required for efficient infection of cells
Teuton JR and Brandt CR
Journal of Virology, 81(8), 3731-3739 (2007)
MicroRNA-183-5p increases with age in bone-derived extracellular vesicles, suppresses bone marrow stromal (stem) cell proliferation, and induces stem cell senescence
Davis C, et al.
Tissue Engineering: Part A, 23(21-22), 1231-1240 (2017)
Mitogen-activated protein kinase phosphatase 1 regulates bone mass, osteoblast gene expression, and responsiveness to parathyroid hormone
Mahalingam CD, et al.
The Journal of Endocrinology, 211(2), 145-156 (2011)
Nicolas Leventoux et al.
Stem cell research, 47, 101896-101896 (2020-07-14)
Amyotrophic Lateral Sclerosis is the most common motor neuron degenerative disease in adults, and TARDBP gene mutations have been reported to be involved in the pathogenesis. We present here how we generated the human induced pluripotent stem cell (hiPSC) line
Aneela Yasmeen et al.
Molecular biotechnology, 58(12), 807-820 (2016-10-21)
The conserved coat or V2 gene of begomoviruses is responsible for viral movement in the plant cells. RNAi technology was used to silence V2 gene for resistance against these viruses in transgenic plants. The transformation of the RNAi-based gene construct
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문서

Colorimetric Phosphatase/Peroxidase Substrate Systems

NBT-BCIP substrate system aids in western blotting and immunohistological staining, producing a blue-purple insoluble end product.

프로토콜

Immunodetection Using BCIP/NBT Substrate

BCIP/NBT substrate produces stable, intense color for alkaline phosphatase assays, ideal for visual observation.

Immunohistochemistry Procedure

Use this protocol to for the entire immunohistochemistry (IHC) procedure through staining and visualization of specific antigens in paraffin-embedded tissue sections.

Guide: Expansion & Differentiation of Human Mesenchymal

TrueGel3D® Hydrogel Plate protocol guides high-throughput culture of human adipose MSCs for screening applications.

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