C1427

Cytochrome b₅ human

recombinant, expressed in E. coli (histidine-tagged), ≥90% (SDS-PAGE)

• 동의어(들): Cytochrome b5
• MDL number: MFCD02687283
• UNSPSC 코드: 12161501
• NACRES: NA.47

속성

• 생물학적 소스: human
• Quality Level: 200
• 재조합: expressed in E. coli (histidine-tagged)
• 분석: ≥90% (SDS-PAGE)
• 형태: solution
• 분자량: 16 kDa
• 기술: inhibition assay: suitable
• 적합성: suitable for molecular biology
• UniProt 수납 번호: P00167
• 배송 상태: dry ice
• 저장 온도: −70°C
• 유전자 정보: human ... CYB5A(1528)

설명

일반 설명

Cytochrome b5 (CYB5A) gene codes for cytochrome b5 protein. It is located on human chromosome 18. It is co-expressed in the Leydig cells of the testis and theca cells of the ovary.

생화학적/생리학적 작용

Cytochrome b5 (CYB5A) mediates NADH-dependent reduction of methemoglobin (erythrocytes), electron transfer to fatty-acid desaturases (mainly liver) and steroid and xenobiotics metabolism by cytochrome P450 enzymes. It is essential for androstenedione synthesis. CYB5A helps in the efficient synthesis of gonadal androgen.

Cytochrome b₅ is a membrane-bound heme-containing protein that enhances the catalytic efficiency of some P450 isoforms. Its mechanism of action is unknown.

물리적 형태

Solution in 20 mM phosphate buffer, pH 7.25, with 0.1 mM EDTA

분석 메모

Heme content measured using difference spectroscopy. An extinction coefficient of 100 mM^-1 for the absorbance difference between 490 nm and 424 nm is used to calculate the concentration of cytochrome b₅.

안전성 정보

• Storage Class Code: 10 - Combustible liquids
• WGK: WGK 1
• Flash Point (°F): Not applicable
• Flash Point (°C): Not applicable
• 개인 보호 장비: Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)