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C5614

Sigma-Aldrich

Cytochrome P450 human

1A2 Isozyme Microsomes, with P450 Reductase and cytochrome b5, recombinant, expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

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Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

human

Quality Level

recombinant

expressed in baculovirus infected insect cells (BTI-TN-5B1-4)

form

solution

mol wt

58 kDa

packaging

vial of 0.5 nmol

UniProt accession no.

application(s)

cell analysis

shipped in

dry ice

storage temp.

−70°C

Gene Information

human ... CYP1A2(1544)

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Application

Human cytochrome P450 has been used in a study to investigate non-viral environmental risk factors for nasopharyngeal carcinoma. Human cytochrome P450 has also been used in a study to investigate the dynamics and hydration of the active sites of mammalian cytochromes.

Biochem/physiol Actions

Cytochrome P450 enzymes are heme containing monooxygenases which are found primarily in the mammalian liver. They catalyze the oxidative metabolism of xenobiotics. This metabolism is the initial step in the biotransformation and elimination of a wide variety of drugs and environmental pollutants from the body. This product has a molecular mass of approximately 58 kDa. Amiodarone, furafylline, and cimetidine are inhibitors, whereas tobacco, insulin, and omeprazole are inducers of the enzyme. The isozyme CYP1A2 is a major pathway for the 6-hydroxylation of melatonin in vivo. This isozyme also catalyzes the 5-hydroxylation of thiabendazole.
Cytochrome P450 is a heterogeneous family of isozymes whose primary function is to oxidize small molecules, both as a function of intermediary metabolism (e.g., fatty acids) and to detoxify exogenous compounds (drugs or toxins). Some isoforms have narrow substrate specificity, while others are promiscuous.

Other Notes

Microsomes containing recombinant human CYP1A2, recombinant rabbit NADPH-P450 reductase, and cytochrome b5.

Physical form

Solution in 100 mM potassium phosphate buffer, pH 7.4.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

ppe

Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)


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