C3142

α-Chymotrypsin from bovine pancreas

(TLCK treated to inactivate residual tryspin activity), Type VII, essentially salt-free, lyophilized powder, ≥40 units/mg protein

• 동의어(들): TLCK-Chymotrypsin
• CAS Number: 9004-07-3
• 효소 위원회 번호: 3.4.21.1 (BRENDA, IUBMB)
• EC Number: 232-671-2
• MDL number: MFCD00130481
• UNSPSC 코드: 12352204
• eCl@ss: 42010112
• NACRES: NA.54

속성

• Quality Level: 200
• 유형: Type VII
• 형태: essentially salt-free, lyophilized powder
• 특이 활성도: ≥40 units/mg protein
• 분자량: 25 kDa
• solubility: 1 mM HCl: soluble 10 mg/mL, clear
• UniProt 수납 번호: P00767
• 저장 온도: −20°C
• 유전자 정보: cow ... CTRB1(618826)

설명

애플리케이션

α-Chymotrypsin from Sigma has been used to determine the crystal structures of two homologous inhibitors (pars intercerebralis major peptide-C and pars intercerebralis major peptide-D2v) from the insect Locusta migratoria by forming a complex with the enzyme.

생화학적/생리학적 작용

α-Chymotrypsin is a serine peptidase and has 241 amino acid residues contained in three polypeptide chains (A chain-13 residues, B chain-131 residues, and C chain-97 residues) linked by disulfide bridges. Molecular weight of this enzyme is found to be 25 kDa. Its pI is 8.75. It selectively hydrolyzes peptide bonds on the C-terminal side of tyrosine, phenylalanine, tryptophan, and leucine. Ca²⁺ activates and stabilizes the enzyme. The enzyme is inhibited by diisopropyl fluorophosphate (DFP), phenylmethanesulfonyl fluoride (PMSF), N-p-tosyl-L-phenylalanine chloromethyl ketone (TPCK), chymostatin, aprotinin, α₁-antitrypsin, α₂-macroglobulin, 10 mM Cu²⁺ and Hg²⁺.

A serine protease that hydrolyzes peptide bonds with aromatic or large hydrophobic side chains (Tyr, Trp, Phe, Met, Leu) on the carboxyl end of the bond.

단위 정의

One unit will hydrolyze 1.0 μmole of BTEE per min at pH 7.8 at 25 °C.

제조 메모

TLCK treatment inactivates trypsin which may be present in chymotrypsin, without affecting the chymotrypsin activity.

분석 메모

Protein determined by A1%/280

기타 정보

View more information on chymotrypsin at www.sigma-aldrich.com/enzymeexplorer

안전성 정보

• 픽토그램: GHS08,GHS07,GHS09
• 신호어: Danger
• 유해 및 위험 성명서: H302,H315,H319,H334,H335,H400
• 예방조치 성명서: P261 - P264 - P273 - P301 + P312 - P302 + P352 - P305 + P351 + P338
• Hazard Classifications: Acute Tox. 4 Oral - Aquatic Acute 1 - Eye Irrit. 2 - Resp. Sens. 1 - Skin Irrit. 2 - STOT SE 3
• 표적 기관: Respiratory system
• Storage Class Code: 11 - Combustible Solids
• WGK: WGK 1
• 개인 보호 장비: dust mask type N95 (US), Eyeshields, Faceshields, Gloves