추천 제품
사용
sufficient for 1,000 multiwell tests (in 96-well multiwell plates)
sufficient for 100 standard tests (1 ml)
Quality Level
배송 상태
dry ice
저장 온도
−20°C
유전자 정보
human ... CASP3(836)
일반 설명
Caspase 3 Colorimetric Assay Kit provides the reagents needed for a quick and efficient detection of caspase 3 activity in cell lysates and in purified preparations of caspase 3.
The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).
The Caspase 3 Colorimetric Assay Kit is based on the hydrolysis of acetyl-Asp-Glu-Val-Asp p-nitroanilide (Ac-DEVD-pNA) by caspase 3, resulting in the release of the p-nitroaniline (pNA) moiety. p-Nitroaniline is detected at 405 nm (εmM=10.5). The concentration of the pNA released from the substrate is calculated from either the absorbance values at 405 nm or from a calibration curve prepared with pNA standards (pNA standard included with the kit).
애플리케이션
The assay can be performed (a) in 1 mL volume and measured using a spectrophotometer, or (b) in 100 µL volume in a 96-well plate using an ELISA reader.
단위 정의
One unit of caspase 3 will cleave 1.0 μmol of Ac-DEVD-pNA per min at pH 7.4 at 25 °C.
기타 정보
Use 17 megohm water only
키트 구성품 전용
제품 번호
설명
- Water (17-18 megaOhm) 125 mL
신호어
Danger
유해 및 위험 성명서
Hazard Classifications
Acute Tox. 3 Dermal - Acute Tox. 3 Inhalation - Acute Tox. 3 Oral - Aquatic Chronic 3 - STOT RE 2
Storage Class Code
6.1C - Combustible acute toxic Cat.3 / toxic compounds or compounds which causing chronic effects
Flash Point (°F)
415.4 °F - closed cup
Flash Point (°C)
213 °C - closed cup
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시험 성적서(COA)
Lot/Batch Number
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문서
Cellular apoptosis assays to detect programmed cell death using Annexin V, Caspase and TUNEL DNA fragmentation assays.
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